5-氟尿嘧啶、表阿霉素、丝裂霉素单用及合用时对胰腺癌细胞Aspc-1和Bxpc-3的抑制作用

Interaction between 5-fluorouracil,mitomycin and epirubicin on human pancreatic carcinoma cell line Aspc-1 and Bxpc-3

目的 :观察 5 氟尿嘧啶 (5 FU)、表阿霉素 (E ADM)和丝裂霉素 (MMC)体外对胰腺癌细胞 (Aspc 1及Bxpc 3)的作用和药物联合应用时的相互作用。方法 :采用MTT测定法观察 5 FU、E ADM和MMC单用及合用时对细胞的抑制作用 ,并应用中效原理判定药物合用的效果。结果 :单用或者合用时随着药物浓度的增加其对细胞的抑制作用也增加 ,5 FU和MMC合用于Aspc 1细胞株 ,当效应为 0 .84时CI为1,大剂量时 (效应大于 0 .84 )是协同作用 (CI <1) ,小剂量时 (效应小于 0 .84 )是拮抗作用 (CI >1)。MMC和E ADM合用时表现为轻度的拮抗作用。 5 FU和MMC、MMC和E ADM合用于Bxpc 3,当效应分别为0 .4 9、0 .6 2时CI为 1,大于此效应表现为轻度拮抗(C >1) ,小于此效应则相反。 5 FU和E ADM合用对两株细胞量效曲线图的形态相似表现为轻度的拮抗作用 ,随着效应的增加拮抗作用略有下降。结论 :药物的相互作用与药物浓度有关 ,药物及细胞株的不同其量效曲线存在较大差异。通过动脉灌注或保留导管持续灌注以提高化疗药物局部浓度能够改善胰腺癌的化疗效果。

AIM: To observe effects of 5-fluorouracil,mitomycin and epirubicin on human pancreatic carcinoma cell line Aspc-1 and Bxpc-3. METHODS: The MTT assay and median-effect principle were used to observe the effects. RESULTS: The effects of the individual or combined drugs were enhanced as drug concentration increased. The interaction between 5-fluorouracil and mitomycin was synergistic at higher concentration (CI<1) and antagonistic at lower concentration (CI>1),but between mitomycin and epirubicin,it was slight antagonistic on Aspc-1. The interactions between 5-fluorouracil and mitomycin,mitomycin and epirubicin were synergistic at lower concentration (CI<1) and antagonistic at higher concentration (CI>1) on Bxpc-3. The interaction between 5-fluorouracil and epirubicin was slight antagonistic on both cell line. CONCLUSION: Increment of the local drug concentration by intraarterial infusion or consecutive arterial infusion through the reserved catheter can improve the chemotherapeutic efficiency of pancreatic carcinoma. The interactions between the drugs are related to the drugs concentration,different drugs and cell line.