川芎嗪注射液对离体豚鼠气管平滑肌作用机制探讨

Effects of tetramethylpyazine injection on isolated tracheal smooth muscles of guinea pigs

目的 :研究川芎嗪对离体豚鼠气管平滑肌的作用及机制。方法 :取豚鼠气管制成气管片 ,用体外实验方法分别记录川芎嗪对豚鼠气管平滑肌的作用以及在钙通道和不同受体阻断剂孵育的条件下对豚鼠气管平滑肌的作用的变化。结果 :川芎嗪对离体豚鼠气管平滑肌有明显的舒张作用 (P <0 .0 1) ,呈剂量依赖性 (10 -5.5～ 10 -3 mol·L-1) ,其作用可以被普萘洛尔 (心得安 )所拮抗 ;其与硝苯地平有协同舒张气管平滑肌的作用 ;M受体阻滞剂阿托品对川芎嗪的作用无明显影响。结论 :川芎嗪对离体豚鼠气管平滑肌有明显的舒张作用 ,其机制很可能是通过作用于气管平滑肌上的 β2 受体 ,抑制细胞外Ca2 +的内流而导致气管平滑肌的舒张。

AIM: To investigate the effects of tetramethylpyazine (TMP) on isolated trachea of guinea pigs. METHODS: The contraction effects were observed from the isolated tracheal smooth muscles. RESULTS: TMP had a significant relaxing effect of concentration-dependent on isolated tracheal smooth muscles and the effect was antagonized by propranolol. It had also obvious synergism to nifedipine in the direct relaxation to the isolated tracheal smooth muscles. Atropine had no effect on the relaxation of the TMP. CONCLUSION: TMP has a significant relaxing effect on isolated tracheal smooth muscles of guinea pigs. The effect is related with β_2 receptors and inhibiting calcium inflow.