摘要
建立一个可以识别牛瘟病毒亚洲株的PCR方法。根据已发表的牛瘟病毒 7个毒株的F基因序列 ,设计合成了一对扩增跨幅为 43 6bp的引物 ,这对引物对日本之中村 系兔化牛瘟病毒感染的 Vero细胞毒和日本之中村 IV系兔化牛瘟病毒感染的兔血毒、组织毒进行 RT-PCR扩增 ,结果该方法对该牛瘟病毒 RNA的扩增取得了与预期大小一致的 RT-PCR产物 ,而对照样品的扩增全为阴性 ,说明具有很好的特异性 ;该方法最低可检测到 0 .1 6ng的牛瘟病毒RNA。
A polymerase chain reaction (PCR)assay for the detection of rinderpest virus of Asian strain has been developed, A pair of primers were designed according to the sequences of F gene of 7 rinderpest reference strains。Using these primers,the 436 bp-long F cDNA products were amplified by RT-PCR from cell products,tissue products and blood products of Rinderpest normal strain;But not from other control samples. indicating this method has good specificity. The sensitivity was assessed by detecting as little as 0.16 ng Rinderpest RNA.
出处
《动物医学进展》
CSCD
2004年第3期93-95,共3页
Progress In Veterinary Medicine
基金
农业部牛瘟普查监测项目
