摘要
为给加速 Vc-獭兔育种提供理论依据 ,利用垂直板聚丙烯酰胺凝胶电泳技术对 Vc- 系、 系獭兔、日本大耳白兔和新西兰白兔血液中的红细胞酯酶 (Es-1、Es-2和 Es-3 )、血清前转铁蛋白 (Prt)、后白蛋白 (Po)等 5个蛋白位点的多态性进行了测定 ,结果表明 :在 4个群体中 ,Es-1、Es-2、Es-3和 Po各有 3种表型 ,分别受 2个共显性等位基因控制 ,Prt有 6种表型 ,受 3个共显性等位基因控制。计算表型频率及基因频率。并根据基因频率计算遗传距离和聚类分析 ,分析结果 :Vc- 系、Vc- 系獭兔的亲缘关系最近 ;Vc-獭兔与日本大耳白兔的亲缘关系较近 ,与新西兰白兔的亲缘关系较远 ,日本大耳白与新西兰白兔的亲缘关系最远。
Polymorphism was detected In five protein loci, including erythrocyte esterase(Es-1,Es-2 and Es-3),pretransferrin(Prt),postalbumin(Po),be using vertical plate polyacrylamide gels electrophoresis in blood samples from Vc-ⅠRex rabbit(Vc-Ⅰ), Vc-Ⅱ Rex rabbit(Vc-Ⅱ), Japanese white rabbit(JWR), New Zealand white rabbit(NZR). Gene frequencies and genetic distance among the four rabbit breeds (or strains) were calculated and the cluster analysis was conducted by using unweighted pair-group arithmetic mean. There were 3 phenotypes in Es-1, Es-2,Es-3 and Po, which were controlled by 2 autosomal codominant alleles; 6 phenotypes in Prt were controlled by 3 autosomal codominant alleles. The frequency of gene and phenotype of the polymorphic protein loci analysed were different among 4 rabbit populations. According to the cluster analysis, the relationship between Vc-Ⅰand Vc-Ⅱ was the nearest; Vc-Ⅰand Vc-Ⅱwere nearer to JWR than to NZR; The relationship between JWR and NZR was the farest. And it was consist with the history of breeding.
出处
《动物医学进展》
CSCD
2004年第3期102-105,共4页
Progress In Veterinary Medicine
基金
国家自然基金 (No.30 170 6 80 )
