射干的毛细管电泳指纹图谱研究

Study on the Fingerprints of Belamcanda chinensis (L.) DC by Capillary Electrophoresis

采用毛细管区带电泳法,以80mmol/L硼酸 15mmol/L硼砂缓冲液(用氢氧化钾溶液调pH9 7)为背景电解质,运行电压12 1kV,检测波长228nm,重力进样10s(高度7cm),建立了射干药材的毛细管电泳指纹图谱(CEFP)。标定CE指纹峰为21个,方法的精密度和重现性较好,相对迁移时间的相对标准偏差(RSD)不大于3 5%,相对峰面积的RSD约为5 0%,10个产地样品的CEFP与标准CEFP的相似度为0 913～0 993。同时,对指纹图谱中各指纹峰确立的方法和各组分含量情况的评价方法进行了初步探讨。

A capillary electrophoresis fingerprints (CEFP) method was established to evaluate the quality of Belamcanda chinensis (L.) DC. The background electrolyte (BGE) was a 80 (mmol/L) boric acid containing 15 (mmol/L) borate solution (adjusted pH 9.7 with KOH solution). The detection wavelength was 228 nm and 12.1 kV was applied. The Belamcanda chinensis (L.) DC was extracted by water to obtain a CEFP containing 21 peaks. The CEFP has good precision, reproducibility and stability with the relative standard deviations (RSDs) of the relative migration time less than 3.5% and the RSDs of the relative peak area about 5.0%. There were good similarities between the standard CEFP and each CEFP of samples collected from ten different places. The coefficients were from 0.913 to 0.993. Meanwhile, fundamental studies related to the methods in peak identification of fingerprints and content evaluation of different sample constituents were carried out.