反相高效液相色谱法测定生物转化体系中的甘草酸

Determination of Glycyrrhizinic Acid in Biotransformation System by Reversed-Phase High Performance Liquid Chromatography

采用高效液相色谱法在HypersilC18色谱柱(4 6mmi d ×250mm,5μm)上以甲醇 水 冰醋酸(70∶30∶1,体积比)为流动相分离测定了甘草酸单铵盐生物(酶)转化体系中的甘草酸,流动相流速为1 0mL/min,紫外检测波长254nm。实验结果表明,该方法在进样量为0 2～20μg时具有良好的线性;样品的加标回收率为98%～103%,相应的相对标准偏差为0 16%～1 58%。方法简便、快速、可靠。

A method for determining glycyrrhizinic acid in the biotransformation system by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The HPLC conditions were as follows: Hypersil C18 column(4.6 mm i.d.×250 mm, 5 μm) with a mixture of methanol-water-acetic acid (70∶30∶1, v/v) as the mobile phase; flow rate at 1.0 (mL/min); and UV detection at 254 nm. The linear range of glycyrrhizinic acid was 0.2-20 μg. The recoveries were 98%-103% with relative standard deviations between 0.16% and 1.58% (n=3). The method is simple, rapid and accurate for determining glycyrrhizinic acid.