超支化聚合物化学键合毛细管电泳柱的制备及其对蛋白质的分离行为

The Preparation of Chemical-Bonded Hyperbranched Polymer Coated Column for Capillary Electrophoresis and Their Application to Separation of Basic Proteins

分别合成了以三羟甲基丙烷和季戊四醇为核的超支化聚(胺 酯),并对其进行了红外测定、羟值测定、粘度测定等表征。采用化学键合方法将其涂于毛细管内壁,并测定涂层柱的电渗流以及对碱性蛋白质的分离能力,结果表明,涂层柱能有效地抑制碱性蛋白质在毛细管内壁上的吸附,大大降低电渗流;以三羟甲基丙烷为核的超支化聚(胺 酯)涂层柱的塔板数达105/m,而以季戊四醇为核的超支化聚(胺 酯)涂层柱的分离柱效更高,塔板数达107/m。实验结果表明这两类涂层柱都具有较好的分离效果和稳定性。

A series of hyperbranched poly(amine-ester)s were synthesized using one-step and pseudo-step procedure respectively, and their molecular structures were characterized by IR, hydroxyl value and viscosity. These polymers were coated on the inner surface of the fused-silica capillaries in a chemical bonding process. The electroosmotic flow and the separation capability for basic proteins of the coated capillaries were investigated. The results demonstrated that the coating in the capillaries could greatly reduce the electroosmotic flow and effectively suppress protein adsorption in the pH range of 3-7. The separation efficiency of the coated capillary of hyperbranched poly(amine-ester)s with trimethylolpropane and pentaerythritol as the core was 10~5/m and 10~7/m, respectively. The separation efficiency and the stability of basic proteins on these coated capillaries were found excellent.