3-苯基乳酸的手性毛细管电泳拆分

Chiral Separation of 3-Phenyllactic Acid by Capillary Electrophoresis

考察了环糊精种类、环糊精浓度、缓冲溶液pH、分离电压、温度等因素对3 苯基乳酸手性分离的影响,并对分离条件进行了优化。结果表明,采用区带毛细管电泳技术,以0 03mol/L的羟丙基 β 环糊精为手性选择剂,0 1mol/L的磷酸缓冲溶液(pH5 5)为电泳缓冲溶液,26kV的分离电压,在25℃下可使3 苯基乳酸对映体达到基线分离,分离度为1 51。

Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 (mmol/L) hydroxypropyl-β-cyclodextrin as the chiral selector, and 0.1(mol/L) phosphate solution (pH 5.5) as the running buffer, the baseline separation of 3-phenyllactic acid enantiomers was achieved with a resolution factor of 1.51. The separation voltage was 26 kV and the temperature was 25 ℃.