HA对牛卵母细胞体外成熟和早期胚胎体外发育影响的研究

Effect of hyaluronic acid on in vitro maturation of bovine oocytes development of early embryos

在TCM-199中添加一些确定的辅助成分,探讨在无血清培养基中添加不同浓度HA对牛卵母细胞体外成熟及早期胚胎体外发育的影响。结果表明,在卵母细胞成熟培养液(TCM-199-m)中添加4.0mg/mL的HA时,卵母细胞的成熟率和卵裂率与对照组(BSA)无显著差异(P>0.05),分别为85.14%,83.57%和89.47%,85.24%,但显著高于其他各浓度组(P<0.05),表明HA在卵母细胞无血清成熟培养中可代替BSA。在受精卵体外培养液(TCM-199-c)中添加HA时,囊胚发育率与对照组(BSA)差异不显著(P>0.05),分别为27.64%和26.51%,但显著高于TCM-199-c组(P<0.05),表明HA对牛胚胎体外发育具有明显的促进作用,在无血清培养中可以代替BSA。当在TCM-199-c+HA中添加少量的BSA时,囊胚发育率(31.19%)显著高于其他各组(P<0.05),表明HA欲完全代替BSA还有不完善之处,有待进一步研究。

Some adjective ingredients were added to TCM-199,through which to determine the effect of Hyaluronic acid (HA) on in vitro maturation (IVM) of bovine oocytes and development of the early embryos.The result showed that,there was no significant difference between control group and the group with 4 mg/mL HA in the frequency of first polar body extrusion (PB) of oocytes and cleavage rate ((85.14%),83.57% VS 89.47%,85.24%,P>0.05);It was significantly higher than other groups in PB and cleavage rate.HA was a substitute for bovine serum albumin (BSA) in serum-free medium to mature the oocytes,there was no significant difference between the group with HA and control group (BSA) ((27.64%) VS 26.51%,P>0.05) in TCM-199-c;but it was significantly higher than other groups ((P<0.05)).HA can improve in vitro development of bovine embryos in serum-free medium,while the groups with HA+BAS demonstrated significantly higher than all the groups in blastocyst rate(31.19%)(P<0.05);BSA can't be replaced completely by HA on in vitro production of bovine embryos in serum-free medium,which is to be studied further.