T细胞疫苗在临床肾移植中的应用

Application of T cell vaccine to clinical renal transplantation

目的探讨T细胞疫苗(TCV)的抗排斥作用及其在临床肾移植中应用的可行性。方法采取经供者抗原(外周血白细胞)致敏的肾移植受者的淋巴细胞在体外经过活化、扩增和灭活,制备T淋巴细胞疫苗,然后将其回注于自身,观察排斥反应发生情况。结果6例患者中,1例患者在输注TCV时出现发热反应。6例患者均未观察到急性排斥反应的发生。淋巴细胞毒及混合淋巴细胞反应在注射TCV前后无明显变化,PHA刺激的淋巴细胞增殖反应在注射TCV后得到增强。结论T细胞疫苗应用于临床器官移植防治排斥反应,无明显副作用,在技术路线上是可行的。

Objective: To investigate the effect of T cell vaccination in prevention of allograft rejection in clinical renal transplantation. Methods: Donor antigen (peripheral white blood cells)-primed renal recipients` lymphocytes were obtained by the method of density gradient centrifugation. The antigen primed-lymphocytes were activated, proliferated and attenuated in vitro for the preparation of T cell vaccine (TCV). TCV was intravenously into the renal recipients before transplantation. Results: Of the 6 cases of renal recipients who received T cell vaccination, 1 case had a fever during the injection of TCV, no acute rejection was observed. T lymphocyte proliferation induced by PHA was increased in the vaccinated patients. The mixed lymphocyte reaction and the antibody-mediated cytotoxicity were not affected by TCV. Conclusions: It is feasible to applicate T cell vaccine to clinical renal transplantation as a preventive for allograft rejection.