用含活性细胞的胶原支架复合皮修复小鼠全层皮肤缺损的研究

Repair of murine full skin loss with composite skin of collagen scaffold containing living cells

目的 构建具有活性细胞成分的复合皮 ,观察其在修复小鼠全层皮肤缺损中的作用。方法 将异种成纤维细胞种植于脱软骨细胞胶原支架上培养 3d ,形成真皮替代物。再将其放置于培养的上皮细胞膜上共同培养 10d ,组成含活性细胞成分的复合皮。将复合皮移植于小鼠的全层皮肤缺损处 ,记录其生长情况并于术后定期活检 ,进行组织学观察。 结果 胶原支架中可见生长良好的成纤维细胞和复层表皮细胞。移植术后 1周 ,复合皮与小鼠缺损创面粘连紧密 ,可见明显的血管化。术后 6周 ,创面愈合良好 ,移植物与创缘融合 ,未见明显排斥反应。 结论 以脱软骨细胞胶原为支架构建的复合皮 ,可作为皮肤替代物修复全层皮肤组织缺损。

Objective To construct composite skin containing living cells and to observe its significance in the repair of full skin loss in mice. Methods The dermal substitute was formed by culturing heterogenous fibroblasts on the acellular chondrocyte collagen scaffold for 3 days, and then cultured on the epithelial membrane for another 10 days, to form the composite skin containing living cells. The composite skin was grafted onto full layer skin defect. The growth condition was observed and biopsies were harvested for histologic examination. Results Both fibroblasts and stratified epithelium grew well in the collagen scaffold. The composite skin adhered tightly to wounds of the mice, with obvious vascularization one week after grafting. The grafts began to merge with the wound margin at 6 post operation weeks without obvious signs of rejection. Conclusion Full skin loss could be repaired by composite skin formed by acellular chondrocyte collagen scaffold as a skin substitute. [