蕈样肉芽肿浸润性皮损中树突细胞表型特征的研究

Phenotype of Dendritic Cells in the Skin Lesions of Mycosis Fungoides

目的 探讨蕈样肉芽肿(MF)皮损中树突细胞(DC)表型及其临床意义。方法 检测DC表面分子的单克隆抗体和免疫组化技术。结果 MF斑片/斑块期的表皮及真皮浅层内存在大量的未成熟DC和成熟DC,主要是CD1a+、CD1c+、Lag+/Langerin未成熟DC和CD83+/DC-Lamp+成熟DC。肿瘤期的真皮内也见大量的CD1a+、CD1c+未成熟DC和CD83+/DC-Lamp+成熟DC,但Lag+/Langerin+DC更多见于表皮和真皮浅层,真皮深层少见,而此处CD1a+、CD1c+未成熟DC明显增多。结论 在MF斑片/斑块期,表皮朗格汉斯细胞发生了迁移,可能参与了抗肿瘤免疫反应,而肿瘤期真皮内大量CD1a+DC可能对相应的免疫耐受产生作用。

Objective To study the characteristics of dendritic cell (DC) phenotype in the skin lesions of mycosis fungoides (MF) and its clinical significance. Methods Immunohistochemistry technique with some monoclonal antibodies against surface molecules on DCs was used. Results There were lots of immature DCs and mature DCs in both epidermis and upper dermis of patch/plaque stage lesions, mainly CD1a+, CD1c+, Lag+/Langerin+ immature DCs and CD83+/DC-Lamp+ mature DCs. In the dermis of tumor stage lesions, there were lots of CD1a+ CD1c+ immature DCs and CD83+/DC-Lamp+ mature DCs, too. However Lag+/Langerin+ cells were shown more in the epidermis and upper dermis, less in the lower dermis, with increasing number of CD1a+, CD1c+ cells in the lower dermis. Conclusions There is migration of epidermal Langerhans'cells in patch/plaque stage of MF lesions, which might take part in anti-tumor immune reactions. Meanwhile, the large amount of CD1a+DCs in the dermis of tumor stage lesions might play a role in the relevant immune tolerance.