银屑病患者外周血干扰素γ和白介素18的表达

Expression of IFN-γ and IL-18 in Peripheral Blood of Psoriasis

目的 探讨进行期银屑病患者外周血干扰素γ(IFN-γ)和白介素18(IL-18)的表达和相关性,及各型银屑病患者外周血IFN-γ的表达变化。方法 进行期寻常性银屑病20例,红皮病性银屑病19例,脓疱性银屑病10例,采用双抗体夹心酶联免疫吸附试验(ELISA)检测血浆中IFN-γ表达水平;对进行期寻常性银屑病患者,检测血浆中IL-18表达水平;检测葡萄球菌肠毒素(SEB)刺激下,进行期寻常性银屑病患者外周血单一核细胞(PBMC)上清液中IFN-γ和IL-18的表达水平。结果 进行期寻常性银屑病患者血浆IFN-γ水平为8.91±2.65,红皮病性银屑病为10.19±5.93,脓疱性银屑病为17.96±10.44,各型银屑病患者均高于正常人(5.70±2.82)。进行期寻常性银屑病患者血浆IL-18水平高于正常人。进行期寻常性银屑病患者PBMC在SEB刺激组IFN-γ和IL-18表达水平均高于空白对照组,且IFN-γ与IL-18表达水平呈显著正相关,r=0.55,P<0.05。结论 在银屑病的发病和进展中,检测IFN-γ的表达量,对早期判断银屑病病情进展、转归和治疗效果有指导意义。IL-18表达增高,推测IL-18可能也参与银屑病发病。

Objective To investigate the expression of IFN-γ and IL-18 in patients with progressive psoriasis and its clinical significance. Methods Twenty cases of psoriasis vulgaris, 19 cases of erythroder-mic psoriasis and 10 oases of pustular psoriasis were studied. Sandwich ELISA was used to detect the plasma level of IFN-γ and IL-18, or supernatant level of IFN-γ and IL-18 in PBMC culture under the stimula-tion of staphylococcal enterotoxin B (SEB). Results The plasma level of IFN-γ in patients with psoriasis vulgaris, erythrodermic psoriasis and pustular psoriasis was higher than that in healthy control. The plasma level of IL-18 in progressive psoriasis vulgaris was higher than that in healthy control. The supernatant level of IFN-γ and IL-18 in PBMC culture in progressive psoriasis vulgaris were higher than that in normal control, with a positive correlation between IFN-γ level and IL-18 level (r = 0.55, P<0.05). Conclusion There is an increasing level of IFN-γ and IL-18 in the patients with progressive psoriasis, which may play an important roles in the pathogenesis of psoriasis.