体外培养正常人黑素细胞内皮素A受体mRNA表达的研究

Expression of ET_(A-R) Gene in Cultured Normal Human Melanocytes

目的 探讨正常人黑素细胞中是否存在内皮素-1的高选择性A受体(ETA-R)的mRNA表达。方法 采用体外黑素细胞培养技术和逆转录-聚合酶链反应(RT-PCR)技术检测正常人黑素细胞ETA-R的mRNA表达情况。结果 正常人黑素细胞有ETA-R的mRNA表达,凝胶电泳显示有特异性条带,以分子量标记电泳条带作为参照,cDNA产物片段长度约为400 bp。结论 内皮素-1可能是通过与其高选择性ETA-R结合而发挥其对黑素细胞的促增殖作用。

Objective To explore the expression of high selective A receptor of endothelin-1 (ETA-R) in melanocytes from normal human beings. Methods Normal human melanocytes were cultured and mR-NA expression of ETA-R in melanocytes was measured by reverse transcription-polymerase chain reaction. Results The mRNA expression of ETA-R was seen in melanocytes. Conclusion This study combined with other experiments suggests that endothelin-1 may affect melanocytes by high selective ETA-R which may con-tribute to the pathogenesis of vitiligo.