粉防己碱对大鼠急性出血坏死性胰腺炎的治疗作用

THE THERAPEUTIC EFFECT OF TETRANDRINE IN TREATMENT OF ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS IN RAT

目的 :观察粉防己碱对大鼠急性出血坏死性胰腺炎的治疗作用。方法 :选用SD大鼠 ,经胆胰管逆行加压注射 4 .5 %牛磺胆酸钠复制急性出血坏死性胰腺炎动物模型 ;化学比色法测血清淀粉酶活性 ;快速比浊法测血清脂肪酶活性 ;湿棉球减干棉球重量测腹水量 ;并观察大鼠 6h存活情况 ;光镜观察胰腺组织病理学改变 ,同时行胰腺炎组织学评分。结果 :6 0mg·kg- 1 和 30mg·kg- 1 粉防己碱使大鼠存活率由 6 5 .2 2 %分别升至 90 .91%和 85 .0 0 % ;腹水量由 3.87± 0 .4 2g分别降至 2 .82± 0 .4 1g和 2 .5 6± 0 .37g;血清淀粉酶由 4 31.78± 74 .0 7u·dl- 1 分别降至 2 70 .0 8±6 6 .85u·dl- 1 和 334.4 8± 5 7.2 5u·dl- 1 ;血清脂肪酶由 74 0 .93± 115 .95IU分别降至 2 76 .70± 87.14IU和 2 37.39±84 .80IU ;与模型组比较均为P <0 .0 5。光镜下可见粉防己碱治疗组胰腺水肿、出血坏死及炎性反应均明显减轻。结论 :粉防己碱对大鼠急性出血坏死性胰腺炎具有较好治疗作用。

Objective: To investigate the therapeutic efficacy of tetrandrine in treatment of experimental acute hemorrhagic necrotizing pancreatitis. Methods: Rat models of acute hemorrhagic necrotizing pancreatitis were induced by retrograde ductal infusion of 4.5% sodium taurocholate. Serum amylase activity was then measured by means of chemical chromatometry. Serum lipase activity was measured by means of swift-turbidimetry. Ascitic fluid was measured via weight difference between wet and dry cotton ball. The effects of tetrandrine on animal survival were measured over 6h, pathological changes of pancreatic tissue were examined via light microscope and acute hemorrhagic necrotizing pancreatitis histological scores were made. Results: In acute hemorrhagic necrotizing pancreatitis rats treated with 60mg·kg -1 and 30mg·kg -1 tetrandrine, the survival percentage was improved from 65.22% to 90.91% and 85.00% respectively; the ascitic fluid was decreased from 3.87±0.42g to 2.82±0.41g and 2.56±0.37g respectively; the serum amylase activity was reduced from 431.78±74.07u·dl -1 to 270.08±66.85 u·dl -1 and 334.48±57.25u·dl -1 respectively; the serum lipase activity was reduced from 740.93±115.95IU to 276.70±87.14IU and 237.39±84.80IU respectively. Under light microscope, morphological findings including edema, acinar necrosis, hemorrhage and inflammatory changes in the pancreas decreased dramatically in the groups treated with tetrandrine. Conclusions: The results suggest a beneficial therapeutic effect of tetrandrine on acute hemorrhagic necrotizing pancreatitis in rats induced by sodium taurocholate.