细胞凋亡表达在食管鳞状上皮细胞癌的临床病理研究

CLINICAL SIGNIFICANCE OF APOPTOSIS IN SQUAMOUS EPITHELIAL CELL CARCINOMA OF ESOPHAGUS

目的 :探讨食管鳞状上皮细胞癌细胞凋亡临床病理意义。方法 :用TUNEL技术免疫组化染色 ,观察30例食管鳞状上皮细胞癌细胞凋亡情况 ,用 10例食管正常鳞状上皮细胞作为对照研究 ,分析它们与食管鳞状上皮细胞癌临床病理因素的关系。结果 :从食管正常鳞状上皮细胞、食管鳞状上皮细胞不典型增生及食管鳞状上皮细胞癌 ,细胞凋亡逐渐减少 ,食管鳞状上皮细胞癌和食管鳞状上皮细胞重度不典型增生的细胞凋亡与食管正常鳞状上皮细胞凋亡比较 ,差异均有显著性 (P <0 .0 1)。结论 :细胞凋亡的异常在食管鳞状上皮细胞癌的发生发展过程中起重要作用 ,且检测细胞凋亡对早期食管癌的判断和食管鳞状上皮细胞癌的病理分级具有指导意义。

Objective: To investigate the clinical significance of apoptosis in Squamous epithelial cell carcinoma of esophagus. Method: The positive expression of apoptotic cells was observed in 30 cases squamous epithelial cell carcinoma of esophagus, using TDT-mediatedx-DUTP nick end labeling technique (TUNEL). 10 cases of normal squamous epithelial cell of esophagus were used as controls. And the relationship of apoptotic cell with clinical pathologic factors was analyzed. Results: The apoptotic cell expression in squamous epithelial cell carcinoma and severe hyperplasia of esophagus was significantly lower than that in normal squamous epithelial cell (P<0.01), and the apoptotic cells were gradually lowered from normal squamous epithelial cell to squamous epithelial cell carcinoma of esophagus. Conclusions: The abnormal apoptotic cells may play an important role in the pathogenesis of squamous epithelial cell carcinoma of esophagus, and the detection of apoptotic cell is of some instructive significance for judging early malignant change of squamous epithelial cell of esophagus and its pathologic grade.