结直肠癌患者外周血CK19 mRNA检测的意义

Study of CK19 mRNA in blood of patients with colorectal cancer

目的:检测结直肠癌患者外周血中上皮性骨架蛋白CK19 mRNA,分析比较其与不同临床病理指标之间的关系,探讨其对临床术后治疗的指导意义。方法:应用RT-PCR方法,选择特异性CK19 mRNA引物,避开假基因CK19b的干扰,检测39例结直肠癌患者外周血中上皮性骨架蛋白CK19 mRNA。用DNA直接测序法排除假基因CK19a的可能。结果:39例外周血CK19 mRNA总检出率为79.5％(31/39),Dukes C、D期(16/16,100.0％)高于Dukes A、B期(15/23,65.2％)(P<0.05)。有淋巴结转移者外周血CK19 mRNA均阳性(16/16),明显高于无淋巴结转移者(65.2％,P<0.05)。而外周血CK19 mRNA的表达与其他临床病理指标无相关性。结论:各期结直肠癌患者外周血均有较高CK19 mRNA表达,随病期进展,阳性率更高。

Purpose: To detect the mRNA expression of epithelial skeleton protein CK19 in the peripheral blood of patients with colorectal cancer; to study its relationship to other clinicopathological parameters; and to discuss if the detection can help post-operative adjuvant therapy. Methods: A total of 39 blood samples from colorectal cancer patients were included in the study. Specific RT-PCR primers were used to avoid the amplification of pseudogenes. Results: Of 39 patients 31 showed CK19 mRNA expression (79.5%) . The patients in Dukes stage C and D showed higher frequency of CK19 mRNA expression than that in Dukes A and B (P< 0. 05). CK19 expression correlated to existing lymph node metastasis. No other clinical parameters related to the expression of CK19 mRNA. Conclusions: CK19 mRNA expression could be detected in all Dukes stages, the frequency in C and D stages were however higher than that in A and B stages.