PDVI患者曲张大隐静脉组织中DMN水平的表达

DMN Expressing Level in the Varicose Great Saphenous Veins of Patients with Primary Deep Vein Valve Insufficiency

【目的】探讨Desmuslin(DMN)及其基因表达水平在原发性下肢深静脉瓣膜功能不全(primary deepvein valve insufficiency,PDVI)引起的大隐静脉曲张组织中的变化。【方法】分别用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)和Western blotting检测PDVI病人曲张大隐静脉以及正常人大隐静脉组织中DMN基因和DMN蛋白的表达水平,用图像分析软件分析电泳照片,比较两组标本条带积分光密度的差异。【结果】RT-PCR中,病例组扩增的条带积分光密度为20 225,3±1 573.6,对照组为117327.9±9183.5(P<0.01);Western blotting中,病例组条带积分光密度为16786.9±3169.3,对照组为93739.3±8614.8(P<0.01)。【结论】DMN蛋白和desmuslin基因表达的下调可能与PDVI引起的大隐静脉曲张有关。

[Objective] To study the alteration of desmuslin (DMN) and the expressing level of its encoding gene in the tissues of varicose great saphenous vein of patients with primary deep vein valve insufficiency (PDVI). [Methods] The desmuslin mRNA level was detected by the method of reverse transcriptase polymerase chain reaction (RT-PCR). The DMN protein was detected by Western blotting. [ Results ] In RT-PCR, the average integrating optimal density of PCR products in patient group was 20 225. 3 ± 1 573. 6, comparing with 117 327. 9 ± 9 183. 5 of the control group. Statistical analysis by t examination indicated significant difference( P < 0. 01). In Western blotting, the average integrating optimal density of the protein bgands in the patient group was 16 786. 9 ±3 169. 3, while that of the control group was 93 739. 3± 8 614. 8 Statistical analysis by t test examination indicated significant difference ( P < 0. 01). [ Conclusion ] Down - regulation of the expressing level of DMN protein and its encoding gene may be a necessary role in the occurrence and development of great saphenous vein varicosis of patients with PDVI.