bFGF及其受体在乳腺癌新生血管中表达及定量分析的实验研究

The Research of Basic Fibroblast Growth Factor and Its Receptor and Quantitative Analysis in Breast Cancer Newly Microvessel

目的:研究bFGF及其受体在乳腺癌间质新生血管中的表达与预后的关系。方法:采用免疫组化、原位杂交和临床随访等方法,观察100例乳腺癌及9例正常乳腺标本中微血管密度(MVD),碱性成纤维细胞生长因子(bFGF)等,并用VIDAS21计算机图象分析系统进行定量和相关性分析。结果:bFGF与bFGF-R在乳腺癌组织的定位,bFGF在正常乳腺组织和癌组织中都有表达,上皮和间质成份均有着色。阳性颗粒分布可见于胞核、胞浆及细胞基质,bFGF在上皮主要位于胞浆,胞核着色,腺体分泌上皮和基底部均有bFGF分布。bFGF在间质主要位于细胞外基质,呈线网状分布,间质细胞也有不同程度的着色。毛细血管内皮细胞均有着色。bFGF-R在正常乳腺组织低表达。癌组织中肿瘤细胞有表达,间质血管有表达,阳性颗粒分布可见于胞核、胞浆,在胞浆中呈线网状分布。bFGF及bFGF-R的图像分析结果,同一标本bFGF和bFGF-R在间质和上皮的分布量大体相当,但无论在乳腺癌上皮还是间质的量均高于正常组织中的量(P<0.01),以癌周最高。肿瘤内微血管密度在组织学分级、淋巴结转移、术后生存期等有显著差异(P<0.01);bFGF-R与微血管密度正相关(r=0.68.P<0.01);bFGF与微血管密度间无相关性。在微血管中表达的bFGF,bFGF-R之间无相关性(r=0.38,P>0.05)。

Objective: To research expression of basic fibroblast growth factor and its receptor in breast cancer newly mi-crovessel. Methods: Observed 100 breast cancer cases,9 normal breast by immunohistochemistry,in situ hybridization technique and clinical investigation. Results:1. The location of bFGF and bFGF-R in breast cancer,bFGF expressed in both normal breast tissue and cancer tissue. bFGF were positively stained, located predominantly in the proper and stroma of both normal breast and breast cancer and overexpression highly. bFGF were seen in the cytoplasm of microvessel endothelium. 2. bFGF receptor were positively stained lowly in normal breast and highly in the proper and stroma of breast cancer(P<0. 01). There was correlation between bFGF receptor expression and tumor grade,stage,lymph node metastasis,tumor size and servival term (P< 0.01). 3. Microvessel density(MVD)was significantly higher than that in node negative cancar(P< 0.01),bFGF-R correlated highly with MVD(r=0.68,P<0.01),bFGF did not correlate with MVD(r=0.38,P>05). Conclusion:bFGF receptor and microvessel density are an important and independent prognostic parameter for breast cancer.