大鼠肠道一过性线虫感染致持续性肠运动功能紊乱

Persistent intestinal motility disorder after transient intestinal nematode infection in rats

目的 :研究SD(Sprague Dawley)大鼠肠道旋毛虫感染及自愈后肠道运动功能 (结肠远端压力测定及胃肠道通过时间 )的变化 ,探讨肠道一过性感染在肠功能紊乱发病中的作用。方法 :云南大理株旋毛线虫幼虫灌胃感染成年雄性SD大鼠 ,利用肠道组织形态学评定肠道炎症的变化和恢复。实验动物分为 :感染后 1 4、4 2、5 6d各组与同龄非感染对照组。所有大鼠均行结肠远端压力测定和胃肠道通过时间检查。结果 :(1 )大鼠旋毛虫感染后 1 4d肠道炎症最重 ,5 6d恢复正常 ;(2 )感染急性期结肠动力活跃 ,无论是静息状态还是给予结肠远端扩张性刺激 ,结肠测压的各项指标均较对照组明显增高 ;(3)感染自愈后结肠在静息状态及小容量 (1mL)扩张刺激时 ,压力变化与对照组比较差异无显著性 ;较大容量 (2mL)扩张刺激时 ,结肠远端收缩时间及收缩波曲线下面积显著增加 ,部分指标与急性期相比有所恢复 ,但仍高于对照组 ;(4 )与对照组比较 ,感染期及自愈后钡条在大鼠肠道分布弥散 ,胃肠道通过时间延缓。结论

Objective: To investigate the intestinal motor function (distal colonic manometry and gastrointestinal transit time) after T. spiralis infection in rats. Methods: Sprague Dawley rats were infected by administering T. spiralis larvae. Rats were studied on 14, 42, and 56 days post infection (PI). Age matched non infected animals served as controls. All rats underwent colonic manometry and gastrointestinal transit time test. Results: (1) The small intestinal inflammation became the most severe on day 14 PI, and returned to normal on day 56. (2) The distal colonic manometry showed significantly active motility in acute infected rats either at rest or upon balloon stimulating. (3) Rat colonic motility parameters were not different from those of the control rats either at rest or upon small volume (1mL) balloon stimulating on day 42 and day 56 PI. But when the balloon was inflated with 2 mL of air, the colonic activity increased significantly compared with that of the control. (4) Gastrointestinal transit time was slower in acute and PI rats than that in the control group. Conclusion: Intestinal motility function was abnormal persistently after transient intestinal nematode infection in rats either in distal colonic manometry or in gastrointestinal transit time.