环氧合酶-2、P-gp在胃癌组织中的表达及其意义

Expression and Significance of Cyclooxygenase-2 Protein and P-glycoprotein in Gastric Carcinoma

目的 探讨环氧合酶 2 (COX 2 )在胃癌组织中的表达及其对P糖蛋白 (P gp)表达的影响。 方法 应用免疫组织化学方法 ,检测 48例胃癌及 10例正常胃组织中COX 2与P gp的表达。 结果 正常胃组织中未见COX 2表达 ,胃癌组织中COX 2表达阳性率为 60 .4% ( 2 9/ 48) ,其阳性表达与肿瘤的TNM分期及淋巴结转移相关 (P <0 .0 5 )。COX 2阳性组中P gp表达阳性率为 82 .8% ( 2 4/ 2 9) ,而COX 2阴性组P gp同时阴性表达率为 63 .2 % ( 12 / 19) ,两者表达呈高度正相关 (γ =0 .470 ,P <0 .0 1)。 结论COX 2表达参与胃癌的发生及恶性进展 ,并可能通过干预P gp的表达参与对肿瘤多药耐药 (MDR)

Objective To investigate the expression of cyclooxygenase-2(COX-2) in gastric carcinoma and its influence on the expression of P-glycoprotein.Methods The expression of COX-2 and P-glycoprotein was examined by immunohistochemical staining in 48 cases of gastric carcinoma and 10 cases of normal controls.Results COX-2 was not expressed in normal gastric tissue.The positive expression rate of COX-2 was 60.4%(29/48) in gastric carcinoma.The expression of COX-2 was correlated with TNM stage and lymph node metastasis(P<0.05).The expression rate of P-glycoprotein was 82.8 %(24/29) in COX-2 positive-expression group.P-glycoprotein was not expressed in 63.2%(12/19) of COX-2 negative-expression group.The expression of COX-2 was significantly correlated with the expression of P-glycoprotein(γ=0.470,P<0.01).Conclusion COX-2 expression is involved in the carcinogenesis and development of gastric carcinoma.It may also play a role in the regulation of multidrug-resistant(MDR) in gastric carcinoma through interfering in the P-glycoprotein expression.