宫颈癌组织中环氧合酶-2表达及其与血管生成和细胞增殖的关系

Expression of Cyclooxygenase-2 and its Relationship with Neovascularization and Tumor Cell Proliferation in Cervical Carcinoma

目的 探讨宫颈癌组织中环氧合酶 2 (COX 2 )表达及其与血管生成和细胞增殖的关系。方法 采用免疫组织化学S P法检测 41例宫颈癌和 10例正常宫颈上皮组织中COX 2表达 ,并检测其微血管密度 (CD3 4标记 )和癌细胞增殖指数 (Ki 67标记 )。结果 宫颈癌组织中COX 2、微血管密度、Ki 67指数明显高于正常宫颈上皮组织 (P <0 .0 1) ,COX 2高表达与宫颈癌淋巴结转移和浸润深度有关 (P <0 .0 5 ) ,与患者年龄、临床分期、组织学类型、分化程度无关 (P >0 .0 5 ) ,COX 2表达阳性者微血管密度和Ki 67指数明显高于COX 2表达阴性者 (P <0 .0 5 )。结论 COX 2过度表达可能参与宫颈癌的发生发展 ,且与宫颈癌新生血管的形成和细胞增殖有密切关系 。

Objective To investigate the expression of COX-2 and its relationship with neovascularization and tumor cell proliferation in cervical carcinoma.Methods The expression of COX-2,microvascular density(MVD)labelled by CD34 protein and proliferation indexes of cancer cells labelled by Ki-67 protein were examined by immunohistochemistry S-P method in 41 cases of cervical carcinomas and 10 cases of normal cervical epithelium.Results The expression of COX-2、MVD、Ki-67 indexes was significantly higher in cervical carcinomas than in normal cervical epithelia(P<0.01).Overexpression of COX-2 in cervical carcinomas was related to lymph node metastasis and depth of invasion,but had no relation to age,FIGO staging,histopathological type and differentiation degree(P>0.05).The MVD and Ki-67 indexes were significantly higher in the COX-2-positive tumors than in the negative ones(P<0.05).Conclusion The expression of COX-2 may play an important role in carcinogenesis and development of cervical carcinoma.It is also closely related with neovascularization and tumor cell proliferation.Detection of COX-2 protein may be valuable to further understand the biological behavior of cervical carcinoma.