期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

牛牙骨质附着蛋白N末端氨基酸序列分析 被引量：2

The N-terminal amino acid sequence analysis of bovine cementum attachment protein
原文传递
导出
摘要 目的 :对牛牙骨质附着蛋白进行N末端氨基酸序列分析 ,为牙骨质附着蛋白的克隆表达提供一定的理论依据。方法 :应用乙酸和盐酸胍提取法获取牛牙骨质附着蛋白 ,鉴定纯度后进行N末端氨基酸序列分析。结果 :所获得的牛牙骨质附着蛋白纯度 >95 % ,N末端 15个氨基酸残基为 :Ile、Pro、Leu、Asp、Pro、Val、Ala、Gly、Cys、Lys、Glu、Pro、Ala、Ala、Asp。 结论 :测得的氨基酸序列具有特异性。 Objective:To analyze the N-terminal amino acid sequence of bovine cementum attachment protein(CAP), which lay a theoretical foundation for cloning and expression of the CAP. Method: Acetic acid and guanidine HCl were selected to extract the bovine CAP. After estimating the purity of CAP, the N-terminal amino acid sequence of CAP was determined. Result: The high purity CAP(>95%) was obtained, and the fifteen N-terminal amino acid sequence were Ile?Pro?Leu?Asp?Pro?Val?Ala?Gly?Cys?Lys?Glu?Pro?Ala?Ala?Asp. Conclusin: The amino acid sequence is possessed of specificity.
作者 吕昕 田宇 肖明振 倪龙兴 李艳君
机构地区 第四军医大学口腔医学院 解放军临潼第一疗养院
出处 《临床口腔医学杂志》 2004年第5期262-263,共2页 Journal of Clinical Stomatology
基金 国家自然科学基金资助 (30 30 0 386)
关键词 牛牙骨质附着蛋白 氨基酸序列分析 bovine cementum attachment protein amino acid sequence analysis
分类号 Q754 [生物学—分子生物学]
  • 相关文献

参考文献7

  • 1Arzate H,Oslon S,Page RC,et al.Production of a monoclonal antibody to an attachment protein derived from human cementum[J].FASEB J,1992,6(14):2990.
  • 2Pitaru S,Narayanan AS,Olson S,et al.Specific cementum attachment protein enhances selectively the attachment and migration of periodontal cells to root surfaces[J].J Periodontal Res,1995,30(5):360.
  • 3Metzger Z,Weinstock B,Dotan M,et al.Differential chemotactic effect of cementum attachment protein on periodontal cells[J].J Periodontal Res,1998,33(2):126.
  • 4Miki Y,Narayanan AS,Page RC.Mitogenic activity of cementum components to gingival fibroblasts[J].J Dent Res,1987,66(8):1399.
  • 5Oslon S,Arzate H,Narayanan AS,et al.Cell attachment activity of cementum proteins and mechanism of endotoxin inhibition[J].J Dent Res,1991,70(9):1272.
  • 6陈晶,付华,陈益,赵玉芬.质谱在肽和蛋白质序列分析中的应用[J].有机化学,2002,22(2):81-90. 被引量：24
  • 7Saito M,Iwase M,Maslan S,et al.Expression of cementum derived attachment protein in bovine tooth germ during cementogenesis[J].Bone,2001,29(3):242.

二级参考文献66

  • 1Roepstorff,P.;Fohlman,J.Biomed.Mass Spectrom.1984,11,601.
  • 2Papayannopoulos,I.A.Mass Spectrom.Rev.1995,14,49.
  • 3Johnson,R.S.;Martin,S.A.;Biemann,K.Int.J.Mass Spectrom.Ion Processes 1988,86,137.
  • 4Senko,M.W.;Speir,J.P.;Mclaffery,F.W.Anal.Chem.1994,66,2801.
  • 5Stimson,E.;Truong,O.;Richter,W.J.;Waterfield,M.D.;Burlingame,A.L.Int.J.Mass Spectrom.Ion Processes 1997,169/170,231.
  • 6Hunt,N.F.;Yates,J.R.;Shabanowitz,J.;Winston,S.;H au er,C.R.Proc.Natl.Acad.Sci.U.S.A.1986,83,6233.
  • 7Gorshkov,M.V.;Ljiljana,P.T.;Smith,R.D.J.Am.Soc.Mass Spectrom.1999,10,15.
  • 8Gu,C.G.;Somogyi,A.;Wysocki,V.H.;Medzihradszky,K.F.Anal.Chim.Acta 1999,397,247.
  • 9Mccormack,A.L.;Somogyi,A.;Dongre,A.R.;Wysocki,V.H.Anal.Chem.1993,65,2859.
  • 10Mabud,M.A.;Dekrey,M.J.;Cooks,R.G.Int.J.Mass Spectrom .Ion Processes 1985,67,285.

共引文献23

同被引文献18

  • 1Somerman MJ, Kagermeir AS, Bowers MR, et al. In vitro attachment of bacteria to extracts of cementum [J]. J Oral Pathology, 1985, 14(10):793 -799
  • 2Arzate H, Oslon S, Page RC, et al. Production of a monoclonal antibody to an attachment protein derived from human cementum [J]. FASEB J, 1992, 6(14):2990-2995
  • 3Arzate H, Jimenez - Garcia LF, Alvarez - Perez MA, et al. Immunolocalization of a human cementoblastoma - conditioned medium-derived protein [J]. J Dent Res, 2002, 81(8):541 -546
  • 4Miki Y, Narayanan AS, Page RC. Mitogenic activity of cementum components to gingival fibroblasts [ J]. J Dent Res, 1987, 66 (8) : 1399 - 1403
  • 5Somerman MJ, Archer SY, Shteyer A, et al. Protein production by human gingival fibrnhlasts is enhanced by guanidine EDTA extracts of cementum [J]. J Periodont Res, 1987, 22(1):75 -77
  • 6Pitaru S, Narayanan SA, Olson S, et al. Specific cementum attachment protein enhances selectively the attachment and migration of periodontal cells to root surfaces [J]. J Periodontal Res, 1995, 30(5) : 360 -368
  • 7Metzger Z, Weinstock B, Dotan M, et al. Differential chemotactic effect of cementum attachment protein on periodontal cells [ J]. J Periodontal Res, 1998, 33(2): 126 -129
  • 8Quarles LD, Yohay DA, Lever LW, et al. Distinct proliferative and differentiated stages of murine MC3T3 - E1 cells in culture: an in vitro model of osteoblast development [ J ]. J Bone Miner Res, 1992, 7 (6) : 683 - 687
  • 9Saito M, Iwase M, Maslan S, et al. Expression of cementum - derived attachment protein in bovine tooth germ during eementagenesis [J]. Bone, 2001, 29(3):242 -248
  • 10Kawskami M, Kuroda S, Yoshida CA, et al. Dental follicle cell - conditioned medium enhances the formation of osteoclast - like multinucleated cells [ J ]. Eur - J - Ortho, 2000, 22 (6) : 675 - 662

引证文献2

二级引证文献8

临床口腔医学杂志
2004年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部