口腔扁平苔藓病损区B_7抗原及CD_(28)的表达及意义

Up-regulated expression of B_7 and CD_(28) in oral lichen planus

目的 :探讨B7/CD2 8共刺激旁路在口腔扁平苔藓 (OLP)病损形成及发展中的作用。方法 :采用免疫组化方法对 46例OLP病损区B7抗原 (B7-1/CD80 ,B7-2 /CD86)及其配体CD2 8的表达情况进行检测 ,并与其局部病变、临床病程及病变类型相联系。结果 :①CD2 8阳性细胞见于所有OLP病损固有层淋巴细胞浸润带中 ,占总浸润细胞数的 45 % -80 %。②CD86低水平表达于正常口腔黏膜上皮及固有层的部分树突状细胞 ,但在不同病程及病变类型OLP病损中均有表达上调 (t =3 4.3 5 ,P <0 .0 1) ;CD86阳性细胞数在有基底层破坏的OLP病损中更多 ,差异有显著性 (t =2 .72 ,P <0 .0 5 )。③OLP病损中CD86阳性细胞与CD2 8阳性细胞之间存在数量上的正相关关系 (γ =0 .946,P <0 .0 1) ;④CD80 低水平表达于 80 .43 %的OLP病损上皮及邻近的结缔组织中 ,但在正常黏膜无表达。结论 :CD2 8-B7共刺激信号涉及OLP病损的形成及发展过程 ;其中 ,口腔黏膜上皮抗原呈递细胞上的CD86与存在于T细胞上的CD2 8分子间的结合及产生的效应 ,在OLP局部破坏性病损的形成中可能起重要作用 ,而CD80 则可能与OLP病损的慢性迁延状态有关。

Objective:It is currently considered that the CD 28 /B 7 costimulatory signal is necessary for the activation and proliferation of T cells. This study is an attempt to elucidate the possible role!of CD 28 /B 7 costimulatory signal in the development of oral lichen planus (OLP) by investigating the expression of B 7-1 (CD 80 ), B 7-2 (CD 86 ) and CD 28 in OLP lesions. Method: Acetone-fixed, frozen tissue sections of 46 cases of OLP were investigated for the expression of CD 80 , CD 86 , and CD 28 by using Immunohistochemical staining and the correlation between the proteins expression and clinical characteristics were analyzed. 10 cases of normal epithelium serve as control.Result:① CD 28 -positive cells appeared in subepithelial band of lymphocytes of OLP and accounted for 45-80% of infiltrated cells. ②CD 86 -positive cells with dentritic shapes were seen in a significantly high numbers in OLP epithelium and connective tissue than in healthy oral mucosa(t =34.35,P<0.01). There were more CD 86 -positive cells in OLP with degenerative changes of basal cellsthanthatwithoutthechanges(t =2 .72 ,P <0 .0 5 ) .③TherewereapositivelycorrelationbetweenthenumberofCD86- positivecellsandCD2 8-postivecellsinOLP(γ =0 .946,P <0 .0 1) .④CD80 -positivecellswereonlysparselyseenin 80 .43 %ofOLPlesions ,whilenotseeninhealthyoralmucosa .Conclusion :TheB7-CD2 8co -stimulatesignalmayin volveinthepathogenesisofOLP .TheeffectofthereactionbetweenCD86onAPCandCD2 8onTcellsmayevoketheim muneresponseandplayaroleintheestablishmentofOLP ,whileCD80 seemstoregulatetheimmuneresponseandaffect thediseasedevelopment ,whichmayberesponsibleforthechroniccliniccharacteristicofOLP .