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SHH对体外培养的小鼠牙乳头间充质细胞增殖的影响

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摘要 目的 观察Sonic Hedgehog对体外培养小鼠牙乳头间充质细胞增殖的影响。方法 构建Shh真核表达载体pCDNA3-Shh,脂质体法转染COS7细胞,收集培养上清波。实验组加入转染pCDNA3-Shh的培养上清液,对照组加入转染空栽体pCDNA3的培养上清液,培养72h,MTT法检测Shh对体外培养的小鼠牙乳头间充质细胞增殖的影响。结果 Shh能促进体外培养的小鼠牙乳头间充质细胞增殖。结论 Shh促进牙孔头细胞增殖,参与牙胚发育。
出处 《中国新医药》 2004年第3期16-17,共2页
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