^(32)P-磷酸铬间质给药在荷人胰腺癌裸鼠的体内生物学分布及形态学表现

Biodistribution pharmacokinetics and toxic effect of P-32 chromic phosphate colloids interstitially injected into human pancreatic cancer (Pc-3)-bearing nude mice

目的 研究32 P 磷酸铬 (32 P胶体 )瘤体间质给药治疗BALB c nu nu裸鼠荷人胰腺癌(Pc 3)移植瘤时在体内相应组织中的分布、药代动力学特点及全身毒性反应。方法 5 1只荷瘤裸鼠 ,经瘤体给予不同剂量32 P胶体或尾静脉给药 ,分批处死 ,动态观察32 P胶体在裸鼠体内放射性分布和组织器官形态学表现 ,观察体重变化和计数WBC和PLT ,测量瘤体表面放射性计数率。结果 32 P胶体瘤体间质注射后其放射性计数率明显高于其他器官组织 ,器官组织放射性计数率瘤体给药明显低于尾静脉给药。瘤体给药有效半减期为 13d。形态学检查显示给药后大部分Pc 3细胞被破坏 ,并出现分化较好的瘤细胞 ;肝、脾、肺及淋巴结等重要器官组织的辐射损伤为可逆性 ,未见明显骨髓抑制现象。结论 32 P胶体瘤体间质给药是治疗胰腺癌安全、简便、有效的核素介入疗法。

Objective To study the biodistribution,pharmacokinetics and toxic effect of chromic phosphate colloids (P-32 colloids) interstitially administered into BALB/c-nu/nu nude mice bearing Pc-3 human pancreatic carcinoma. Methods Fifty-one nude mice were injected with various doses of P-32 colloids through caudal vein or into the tumor by interstitial injection.The radioactive distribution of P-32 colloids in mouse bodies was observed dynamically and white blood cells and platelets were counted,body weight and morphology were studied,and the tumor surface radioactive counts were also measured. Results After intratumoral administration of P-32 colloids,the radioactive counts per minute in the tumor were obviously higher than those in other tissues or organs,and the radioactive counts per minute of other tissues or organs were evidently lower than those after caudal vein administration.The effective half life of P-32 colloids injected was 13 days.Morphological examination demonstrated that most of Pc-3 cells were destroyed after intratumoral administration of P-32 colloids,and differentiated benign tumor cells were detected.The radiation lesions of liver,spleen,lung,lymph node and other important tissues or organs were reversible.No obvious bone marrow depression was detected. Conclusion It is demonstrated that the intratumoral injection of P-32 colloids is a safe,easy and effective radionuclide interventional therapy for pancreatic tumor. ;