摘要
The mechanism of attachment and leaching of thiobacillus ferrooxidans（T.f.） on chalcopyrite were studied. The shaking flasks with bacteria were observed by SEM. The process of T.f attached to the surface of the mineral sample and the biofilm forming were described. The promoting role of the biofilm for bioleaching was discussed. The existence of Fe2+ in the exopolysaccharide layer of T.f was demonstrated by EM（electronic microscope）cell-chemistry analysis. These results show that under the proper growth condition of bacteria, bioleaching of chalcopyrite results in the formation of complete biofilm after 23 weeks. There are iron ions in the outer layer polymer of T.f., which provides the micro-environment for themselves, and can guaruntee the energy needed for the bacteria growth in the biofilm. At the same time, Fe3+ions produced oxidize sulfide which brings about the increase of both growth rate of the bacterial and leaching rate of sulfide minerals.
The mechanism of attachment and leaching of thiobacillus ferrooxidans(T.f.) on chalcopyrite were studied. The shaking flasks with bacteria were observed by SEM. The process of T.f attached to the surface of the mineral sample and the biofilm forming were described. The promoting role of the biofilm for bioleaching was discussed. The existence of Fe^(2+) in the exopolysaccharide layer of T.f was demonstrated by EM(electronic microscope)cell-chemistry analysis. These results show that under the proper growth condition of bacteria, bioleaching of chalcopyrite results in the formation of complete biofilm after 23 weeks. There are iron ions in the outer layer polymer of T.f., which provides the micro-environment for themselves, and can guaruntee the energy needed for the bacteria growth in the biofilm. At the same time, Fe^(3+)ions produced oxidize sulfide which brings about the increase of both growth rate of the bacterial and leaching rate of sulfide minerals.
出处
《中国有色金属学会会刊：英文版》
CSCD
2004年第2期383-387,共5页
Transactions of Nonferrous Metals Society of China
基金
Project(2 0 0 2 0 5 3 3012)supportedbytheDoctoralFoundationofEducationalAdministration,China
project(5 992 5 412)supportedbyNationalNaturalScienceFoundationforDistinguishedYoungScholarofChina
关键词
噬硫杆菌
黄铜矿
生物滤化
膜滤
选矿
bioleaching
bacterial attachment
biofilm forming
EM cell-chemistry
