慢性粒细胞白血病cyclin D2基因的表达研究

Cyclin D2 expression in chronic myelogenous leukemia

目的 研究cyclinD2基因表达与慢性粒细胞白血病 (CML)特征性的P2 10 BCR ABL蛋白酪氨酸激酶活性之间的相关性。方法 以RT PCR、免疫印迹法及流式细胞术检测cyclinD2基因在BCR ABL +K5 6 2细胞系中的表达。通过表达抗ABL蛋白酪氨酸激酶区胞内抗体的模型即K5 6 2 ib eGFP细胞 ,检测cyclinD2表达的变化。结果 在P2 10 BCR ABL 蛋白酪氨酸激酶活性受抑制的K5 6 2 ib eGFP细胞中 ,cyclinD2的表达 (18.90 % )明显低于K5 6 2细胞组 (48.10 % ) ,且K5 6 2 ib eGFP组S期细胞比例(40 4 0 % )也明显低于K5 6 2细胞组 (6 4 .34% )。结论 cyclinD2可能是CMLP2 10 BCR ABL 蛋白酪氨酸激酶下游相关信号传递分子 ,其表达增高可能促进了CML细胞的过度增殖。

Objective To investigate the relationship between cyclin D2 and P210 BCR/ABL tyrosine kinase in chronic myelogenous leukemia(CML). Methods RT-PCR,Western blot and flow cytometry were performed to detect the expression of cyclin D2 in K562 cells and in K562-ib-eGFP cells which express intracellular single-chain antibody (sFv, intrabody) against ABL tyrosine kinase domain. Results Cyclin D2 expression in K562-ib-eGFP cells was 18.90% which was lower than that of control K562 cells(48.10%),and the number of S-phase cells in K562-ib-eGFP was 40.40% which was much lower than that in K562 cells(64.34%). Conclusion Cyclin D2 is a potential down-stream signal molecule of the p210 BCR/ABL tyrosine kinase in CML. The altered expression of cyclin D2 may contribute to the over proliferation of CML cells.