高强度聚焦超声体外辐照兔股动脉后的病理学观察

Pathological study of rabbit femoral after high-intensity focused ultrasound irradiation in vivo

目的 探讨高强度聚焦超声 (HIFU)体外辐照兔股动脉后血管的破坏情况。方法 HIFU的工作频率为 0 .8MHz ,焦距 13 5mm ,焦点平均强度为 80 0 0W /cm2 。 3 0只兔随机分为三组 ,采用弹力纤维维多利亚蓝和丽春红S组织化学染色方法 ,观察HIFU辐照兔股动脉后即日、3d和 7d的病理学变化。结果 HIFU辐照后 ,各实验组均可见兔股动脉血管内皮细胞脱落、消失 ,管腔有微小血栓形成 ;平滑肌细胞出现核固缩 ,细胞排列紊乱 ;血管弹力纤维出现崩溃和断裂等现象。结论 HIFU体外辐照可以造成兔股动脉血管损伤 ,并导致血流动力学改变。

Objective To inquire into the hurt-efforts to the rabbit femoral in vivo irradiated by high intensity focused ultrasound(HIFU). Methods Using a HIFU with the operating frequency of 0.8 MHz; the focal distance of 135 mm and the sound-intensity of 8000 W/cm 2. The average interal diameter of the rabbit femoral artery was about 1.1 mm.Thirty rabbits were divided into 3 groups randomly and unilateral-femoral was chosen at random. To observe by using the method of pathology and victorian blue of elastic fibre. Results After irradiation all the endothetial cells of blood vessels of the rabbit peeled off,disappeared and tiny thrombuses were formed in the vessels the core of smooth muscles withered, cell disorder appeared. Conclusions Irradiation by HIFU could cause injury to the rabbit femoral artery, change blood dynamics and produced contribution to the further research on the energy accumulation of the targets in HIFU system.