卵圆细胞参与实验性肝癌形成过程的研究

The experimental study on the oval cells participating in hepatocarcinogenesis

目的 探讨卵圆细胞在肝组织内的起源及其与原发性肝细胞肝癌的关系。方法 清洁型SD大鼠 6 0只 ,随机分为正常组 (2 0只 )和实验组 (40只 ) ,各组于开始制造肝癌模型后的不同时段取肝组织标本进行常规病理和C kit、PCNA免疫组化检测。结果 正常组大鼠肝脏表面光滑 ,组织学形态正常 ,偶见C kit和PCNA阳性细胞 ;实验组于肝脏染毒的第 2周 ,首先于汇管区发现卵圆细胞沿胆管上皮依次排列增生 ,这些卵圆细胞呈C kit和PCNA阳性表达。随着染毒加重 ,卵圆细胞以汇管区为中心向肝小叶穿插生长。肝癌形成时 ,癌结节内外均见有卵圆细胞聚集 ,此期C kit阳性细胞仍以汇管区为主 ,而PCNA阳性细胞遍布癌结节内外。结论 卵圆细胞起源于汇管区的胆管上皮 ;卵圆细胞与肝癌的发生密切相关。

Objective To explore the genesis of the oval cells in hepatic tissue and relationship between the hepatic oval cells and the primary hepatocarcinoma. Methods Sixty SD rats were divided into normal group (20 cases) and experimental group (40 cases). C-kit and PCNA were continuously detected by immunohistochemistry and the liver pathologic changes were observed by optical microscope in the different period. Results The hepatical surface was lubricous and the histological morpha was normal in the normal group,and the positive cells of C-kit and PCNA were found on occasion. In the experimental group,the oval cells which express C-kit and PCNA were firstly discovered in the periportal regions in the second weeks,and these cells proliferate in turn and rangely in the bile duct epithelium. With the hepatic injury becoming more serious,the oval cells extended into the centrilobular regions from periportal regions. When HCC occurred,the oval cells were found in the cancer nodule and in the margin. In this period,the most of positive cells of C-kit still located in the periportal regions,while the PCNA cells were found in and out of the cancer nodule.Conclusions The oval cells originate from bile duct of the periportal regions. The oval cells play an important role in the process of hepatocarcinogenesis.