幽门螺杆菌感染在胃黏膜病变中三种基因表达及相互关系的研究

Expression and relationship among CD44V6, p16 and PCNA in gastric mucosal lesions with Helicobacter pylori infection

目的 研究幽门螺杆菌 (HP)感染胃黏膜病变中细胞表面分子 (CD4 4V6 )、p16基因及细胞核增殖抗原 (PCNA)的表达及相互关系。方法 114例病人经胃镜及病理证实的不同胃黏膜病变标本 ,应用免疫组化染色法检测CD4 4V6、p16基因及PCNA基因的表达。HP感染由快速脲酶试验结合Warthin Starry银染色确定。结果 各组胃黏膜病变中HP阳性的病人与HP阴性的病人相比较 ,HP阳性的病人p16基因表达显著低于HP阴性的病人 (P <0 0 1) ;CD4 4V6表达HP阳性的病人显著高于阴性的病人 (P <0 0 1) ;HP阳性的病人PCNA LI%表达显著高于HP阴性的病人 (P <0 0 5 )。HP感染的胃黏膜病变中p16基因表达与CD4 4V6表达呈负相关 (rs=- 0 6 971,P <0 0 1) ;p16基因表达与PCNA表达呈负相关 (rs=- 0 6 0 75 ,P <0 0 1) ;CD4 4V6表达与PCNA表达呈正相关 (rs=0 4 799,P <0 0 1)。结论 CD4 4V6、PCNA、p16基因在胃黏膜病变、癌前病变向胃癌转化中起了非常重要作用。HP感染直接或间接的促进了CD4 4V6、PCNA的表达 ,并抑制了p16基因的表达。HP感染患者 3种基因之间存在一定相关性。

Objective To study the expression and relationship among CD44V6,p16 and PCNA in gastric mucosal lesions with HP infection. Methods Expression of CD44V6,p16 and PCNA were investigated in 114 gastric mucosal lesions by use of immunohistochemistry.HP was examined by both Warthin -Starry method and RUT. Ruselts Comparing HP positive group (P<0.01) with HP negative group in gastric mucosal lesions,The expression of p16 in HP positive group were lower (P<0.01);the expression of CD44V6 and PCNA in HP positive group was higher (P<0.05). The expression of p16 were significantly correlated with the expression of CD44V6 ingastric mucosal lesions with HP infection(r s=-0.697 1,P<0.01); the expression of p16 were correlated significantly with the expression of PCNA in gastric mucosal lesions with HP infection,too(r s=-0.607 5,P<0.01); the expression of CD44V6 were correlated significantly with expression of PCNA in gastric mucosal lesions with HP infection (r s=0.479 9,P<0.01). Conclusions The expression of p16,CD44V6 and PCNA plaed an important role in gastric mucosal lesions. It may be a mlaignant transformation from pre-carcinoma to cancer in HP positive patients.HP infection directly or in directly promotes the expression of CD44V6 and PCNA,and suppresses the expression of p16.Because of HP infection,the expression of CD44V6,p16 and PCNA were correlated with each other.