重组蛋白与合成肽抗原对戊型肝炎的诊断价值比较

Comparison of the diagnostic implication between ELISA by recombinant protein and synthetic peptides for detecting antibody to hepatitis E virus

目的 比较重组蛋白与合成肽抗原建立的酶联免疫吸附试验 (ELISA)在戊型肝炎实验室诊断中的应用价值。方法 选择戊型肝炎病毒第二开放读码框架 (ORF2 ) ,分别采用重组蛋白及合成肽抗原 ,建立酶联免疫吸附试验 (ELISA) ,同时检测 97份戊型肝炎急性期患者 ,4 7份甲、乙、丙、丁、庚型肝炎患者及 12 0份健康献血者的血清抗 HEV。结果 重组蛋白ELISA、合成肽ELISA与Genelabs重组抗原ELISA试剂盒检测结果的总符合率分别为 96 .9%和 92 % ;重组蛋白ELISA与合成肽ELISA的一致率为 91% ;合成肽ELISA对重组蛋白ELISA的灵敏度为 90 % ,特异度为 10 0 %。结论 戊型肝炎病毒相同表位的重组蛋白和合成肽抗原用于抗 HEVELISA检测有较好的一致性。

Objective To evaluate the diagnostic implication of ELISA by recombinant protein or synthetic peptides for detecting antibody to hepatitis E virus (HEV). Methods Based on recombinant protein or synthetic peptides from the open reading frame (ORF) 2 of HEV, enzyme-linked immunosorbent assay (ELISA) was developed separately for detecting anti-HEV among 97 sera of acute-phase patients with hepatitis E.and 47 sera of patients with hepatitis A,B,C,D or G Results As comparing with Genelabs ELISA kit,the coincident rate for recombinant protein ELISA was 96.9% and for synthetic paptides ELISA 92%.The consistent rate between recombinant protein ELISA and synthetic peptides ELISA was 91%. Compared to recombinant protein ELISA and synthetic peptides ELISA showed 90% of sensitivity and 100%of specificity.Conclusion Used as a diagnostic antigen in anti-HEV ELISA test, the recombinant protein is identical with the synthetic peptides which come from the same epitope region.