c-kit基因突变对胃肠道间质瘤预后的影响

Effect of c-kit gene mutation on prognosis of gastrointestinal stromal tumor

目的 探讨c kit基因突变与胃肠道间质瘤 (GIST)临床病理指标和预后的关系 ,为GIST恶性变判断、预后评估提供客观指标。方法 对 82例GIST患者运用聚合酶链反应 单链构象多态性分析、DNA测序的方法筛选c kit基因突变病例 ,采用统计学方法回顾性分析c kit癌基因突变与GIST临床病理特征、生物学行为、复发和病死率的关系。结果 c kit基因突变阳性组与c kit基因突变阴性组间肿瘤大小 (χ2 =16 795 ,P <0 0 1)、增殖细胞核抗原指数 (χ2 =17 0 99,P <0 0 1)、有丝分裂计数 (χ2 =11 2 33,P <0 0 1)、坏死 (χ2 =4 4 0 4 ,P <0 0 5 )、浸润 (χ2 =2 2 391,P <0 0 1)、复发 (χ2 =16 333,P <0 0 1)、转移 (χ2 =12 96 5 ,P <0 0 1)、死亡 (χ2 =11 6 4 6 ,P <0 0 1)差异有显著意义 ,而年龄 (χ2 =0 0 0 0 2 ,P >0 0 5 )、性别 (χ2 =0 14 1,P >0 0 5 )、肿瘤的部位 (χ2 =7 5 2 4 ,P >0 0 5 )、细胞类型 (χ2 =0 82 7,P >0 0 5 )、囊性变 (χ2 =1 6 0 8,P >0 0 5 )、出血 (χ2 =0 6 33,P >0 0 5 )、c kit蛋白表达 (χ2 =0 0 0 0 ,P >0 0 5 )差异无显著意义。结论 c

Objective To evaluate the clinical signification of c kit gene mutation in gastrointestinal stromal tumor (GIST) and examine whether the presence of mutation of c kit gene is important as a prognostic factor. Methods The c kit mutation had been detected by PCR SSCP,DNA sequence, statistical comparison were used for the relationship of c kit gene mutation and clinical pathology, clinical behavior, recurrence, et al. Results The presence of c kit mutation correlated with tumor size, proliferating cell nuclear antigen index, mitotic cell number, presence of necrosis, microscopic invasion to adjacent tissues, recurrence and distant metastasis. The age, sex, location of tumor, cell type, the presence of hemorrhage,and c kit expression were independently related to the presence of c kit mutation. Conclusions The c kit gene mutation is an important prognostic factor for GIST.