毫米波对人角质形成细胞缝隙连接通讯功能的影响

Effects of millimeter wave on gap junctional intercellular communication in human keratinocytes

目的 研究低功率毫米波对人皮肤角质形成细胞 (HaCaT)缝隙连接通讯 (GJIC)的影响。方法 采用荧光淬灭后恢复技术 ,用激光共聚焦显微镜检测频率为 30 16GHz、功率密度分别为1 0和 3 5mW/cm2 毫米波辐照对细胞缝隙连接通讯功能的影响。结果 12 氧 14 酰佛波酯 (TPA)5 μg/L可抑制细胞GJIC功能 ,激光淬灭后平均荧光恢复率由正常对照的 (5 5± 17) %降至 (34±13) % ,差异有非常显著性。功率密度为 1 0和 3 5mW /cm2 毫米波单独辐照不改变细胞GJIC功能 ,其平均荧光恢复率分别为 (5 2± 16 ) %和 (5 0± 17) % ;当毫米波与TPA联合作用时 ,TPA诱导的细胞GJIC功能抑制被削弱 ,其中 1 0mW /cm2 毫米波使TPA诱导的细胞GJIC功能抑制部分恢复 ,其平均荧光恢复率为 (47± 16 ) % ,差异有非常显著性 ;而 3 5mW /cm2 毫米波使细胞GJIC功能完全恢复至正常对照水平 ,其平均荧光恢复率为 (5 0± 16 ) % ,差异有非常显著性。结论 毫米波辐照可消除或减小TPA诱导的细胞GJIC功能抑制。

Objective To explore the effect of millimeter wave (MW) at low power density on gap junctional intercellular communication (GJIC) in human keratinocytes (HaCaTs). MethodsFluorescence recovery after photobleaching (FRAP) technique was employed to determine effect of 30.16 GHz MW exposure at 1.0 and 3.5 mW/cm 2 on GJIC with laser confocal scanning microscope. ResultsFRAP analysis revealed that 12-O-tetradecanoylphorbol-13-acetate (TPA) at a dose of 5 μg/L could inhibit GJIC in HaCaTs. Fluorescence recovery rate fell from (55±17)% in the controls to (34±13)% after photobleaching, with a very significant difference ( P <0.001). Exposure to MW alone for one hour at either 1.0 mW/cm 2 or 3.5 mW/cm 2 did not affect GJIC, with fluorescence recovery rates of (52±16)% and (50±17)%, respectively. GJIC suppression induced by TPA was weakened by MW combined with 5 μg/L TPA treatment for one hour, which could be partially recovered by exposure to 1.0 mW/cm 2 MW with fluorescence recovery rate of (47±16)%, P <0.01, and fully recovered by exposure to 3.5 mW/cm 2 MW with fluorescence recovery rate of (50±16)%, P <0.001, with a very significant difference. ConclusionsGJIC suppression induced by TPA could be eliminated or diminished by exposure to millimeter wave in HaCaTs.