增生性瘢痕和瘢痕疙瘩组织中Ⅰ、Ⅲ型胶原蛋白含量的改变及其意义

Content Changes of Type Ⅰ/Ⅲ Collagen in Tissues of Hypertrophic Scar and Keloid and Its Clinical Significance

【目的】了解增生性瘢痕和瘢痕疙瘩组织中胶原蛋白含量及组成的改变。【方法】以羟脯氨酸测定反映Ⅰ、Ⅲ型胶原蛋白总量。SDS PAGE电泳分离proα1 (Ⅰ )及 proα1 (Ⅲ )链 ,考马斯亮蓝染色 ,图像分析仪计算Ⅰ、Ⅲ型胶原蛋白比。【结果】增生性瘢痕和瘢痕疙瘩组织中胶原蛋白含量相近 ,分别为 (0 .988± 0 .1 4 1 )mmol/L和 (1 .1 0± 0 .1 5 9)mmol/L ,均明显高于正常瘢痕和正常皮肤 ;瘢痕疙瘩Ⅰ、Ⅲ型胶原蛋白比值为1 1 .4 91± 1 .2 2 3,高于增生性瘢痕、正常瘢痕和正常皮肤 ,而增生性瘢痕与正常瘢痕和正常皮肤相近。【结论】增生性瘢痕和瘢痕疙瘩组织中各型胶原蛋白表达强度的不一致性 。

To understand the changes of collagen contents and composition in tissues sampls of hypertrophic scar and keloid.The total contents of type Ⅰ/Ⅲ collagen were reflected from estimation of hydroxyproline (pro). The proα1(Ⅰ) and proα1(Ⅲ) collagen chains were separated by SDS-PAGE, then their polypeptides were visualized by staining with Coomassie Brilliant Blue and the ratio of collagen Ⅰ/Ⅲ was calculated with the help of computer color image analysis system.The total contents of collagen in tissues of hypertrophic scar and keloid were very close (0.988±0.141 vs. 1.10±0.159 mmol/L, respectively), all higher than those in tissues of normal scar and healthy skin ; while the ratio of collagen Ⅰ/Ⅲ in keloid tissues was 11.491±1.223, higher than those in hypertrophic scar, normal scar and healthy skin ; whereas that in tissues of hypertrophic scar was more or less similar to those of normal scar and healthy skin.[Conclusion]Keloid and hypertrophic scar tissues display various expression intensity of different types of collagen.This result possesses certain potential significance for pathogenesis elucidation of hypertrophic scarring and keloid as well as differentiation between these two diseases process.