乳腺癌前哨淋巴结淋巴细胞亚群的初步研究

Preliminary study on lymphocyte subsets of sentinel lymph nodes in breast cancer patients

目的 研究乳腺癌发生转移和未发生转移时前哨淋巴结 (sentinellymphnode,SLN)的免疫功能。方法 采用流式细胞技术 ,检测乳腺癌患者SLN中免疫细胞CD3+ T、CD4 + T、CD8+ T、CD16 +NK和CD19+ B的改变 ,并与非SLN的检测结果进行比较。结果 肿瘤未发生转移时 ,SLN的CD3+ T、CD4 + T、CD8+ T、CD16 + NK、CD19+ B数量与非SLN差异无显著性。当肿瘤发生转移时 ,SLN的CD4 + T、CD8+ T发生显著性改变 ,由正常的以CD4 + T(6 9.0 7± 5 .0 2 )细胞为主 ,转变为以CD8+ T细胞 (6 6 .15±5 .97)为主 ;而CD3+ T、CD16 + NK、CD19+ B与肿瘤未发生转移的SLN相比 ,差异无显著性。结论 SLN免疫细胞数量与非SLN相比 ,差异无显著性。但肿瘤细胞转移后 ,改变了淋巴结的微环境 ,使淋巴结内CD4 + T、CD8+ T免疫细胞比例发生改变。

Objective To study the lymphocyte subsets of sentinel lymph nodes (SLN) in breast cancer patients. Methods Flow cytometry was used to analyze the markers on the surface of lymphocytes such as CD3,CD4, CD8,CD16 and CD19 in the sentinel lymph node of breast cancer. Results When lymph node metastasis did not occur,there was no significant difference in the number of CD3 +T,CD4 +T,CD8 +T,CD16 NK and CD19 B cells between SLN cells and non-SLN cells. With lymph node metastasis,the proportion of CD4 + and CD8 +T cells was significantly changed,CD8 +T cells (66.15±5.97 ) were the predominant cells instead of CD4 +T cells(69.07±5.02),whereas no significant difference in CD3 +T,CD16 NK and CD19 B cells. Conclusion The CD4 + to CD8 +T cell ratio in sentinel lymph nodes with metastasis is reversed in breast cancer patients. This might results from changes in microenvironment due to tumor cell invasion.