人白血病不同致瘤潜能细胞系的基因表达差异分析

Differential gene expression analysis of human leukemic cell line with different tumorigenic potentials in nude mice

目的 探索人白血病细胞系在裸鼠体内致瘤的分子生物学机制。方法 应用DNA芯片技术 ,检测K5 6 2和K5 6 2 n细胞的基因表达差异。结果 K5 6 2和K5 6 2 n细胞表达差异显著的基因有 139条 ,其中在K5 6 2 n细胞中表达上调的有 4 2条 ,表达下调的有 97条。在这 139条基因中 ,有 85条已在GeneBank中登录 ,5 4条为新序列。这些基因可以划分为 5个主要的功能群 :(1)肿瘤相关基因 ,包括原癌基因和肿瘤抑制基因 ;(2 )与转录调节、细胞周期、凋亡相关的基因 ;(3)与细胞骨架和细胞动力学相关的基因 ;(4)与物质代谢和转运相关的基因 ;(5 )与免疫功能相关的基因。另外还有一些功能混杂的基因和功能未知基因的表达变化。结论 K5 6 2和K5 6 2 n细胞存在多方面基因的表达差异 ,人白血病细胞系K5 6 2 n在裸鼠体内的高致瘤性与其特异的基因表达谱有关。

Objective To study the molecular mechanism of tumorigenicity in nude mice of human leukemia cell lines. Methods K562-n,is a human leukemic cell line with much higher tumorigenecity in nude mice compared with the parental K562 cell line by repeated in vitro and in vivo passages. Genes differentially expressed between K562 and K562-n cells were analyzed by using DNA microarray technique. Results Of 12 000 genes screened were differentially expressed significantly,among which 42 genes were up-regulated and 97 genes were down-regulated in K562-n cells. Eighty-five of the 139 genes have been registered in the GeneBank and 54 are unknown genes. The genes accessible from the GenBank include: 1. oncogenes and tumor-supressor genes,2. genes related to transcription regulation,cell cycle and apoptosis,3. genes related to cytoskeleton and cytokinetics,4. genes related to metabolism and transport,5. genes related to immune function. There were also some differentially expressed genes with mixed functions and some with unknown function differentially expressed. Conclusion There are many genes differentially expressed between K562-n and K562 cells. The high tumorigenicity in nude mice of human leukemia cell line K562-n might be related to its specific gene expression profile.