肝癌腹水脱落细胞端粒酶活性检测及临床意义

Determination of telomerase activity of exfoliated cells in ascites from patients with hepatocarcinoma and its clinical significance

目的 研究肝癌腹水脱落细胞端粒酶活性。方法 应用银染TRAP法检测肝癌腹水和非癌性腹水脱落细胞端粒酶活性,并将检测结果与细胞学诊断结果进行比较。结果 50例肝癌腹水脱落细胞37例阳性(74.0％),29例非癌性腹水脱落细胞无1例阳性。结论 结果提示端粒酶活性检测可能在癌性腹水诊断和鉴别诊断方面有重要辅助诊断价值。

Objective To study the telomerase activity of exfoliated cells in ascites from patients with hepatocarcinoma by silver-staining telomere repeat amplification protocol. Methods Telomerase activity was analyzed in exfoliated cells in the ascites from 50 patients with hepatocarcinoma and 29 patients with other disease by silver-staining telomeric repeat amplification protocol. Results The positive rate of telomerase activity of exfoliated cells was 74.0% in ascites from patients with hepatocarcinoma and 0% in ascites from patients with other diseases. Conclusion Telomerase is a useful marker for distinguishing ascites from patients with malignant neoplasm from ascites from patients with other diseases.