实验性肾病综合征中的胰岛素样生长因子-1

Insulin-like Growth Factor-1 in the Experimental Nephrotic Syndrome

目的 :了解实验性阿霉素肾病综合征 (NS)的大鼠血清、尿及肝脏组织中胰岛素样生长因子 -1(IGF -1)浓度的变化 ,探讨肾病综合征生长障碍的发病机制。方法 :检测Wistar系雄性大鼠阿霉素致肾病综合征组和正常对照组的24h尿蛋白排泄量和血、尿、肝组织的IGF -1水平。IGF -1的测定采用放射免疫法。结果 :与正常对照组相比 ,肾病组大鼠血清IGF -1明显降低 (P<0.01) ;尿IGF -1明显升高 (P<0.01) ;两组肝组织内的IGF -1浓度差别无统计学意义 (P>0.05)。尿IGF -1浓度与血清IGF -1浓度呈负相关 (r= -0.78,P<0.01),而与24h尿蛋白排泄量呈正相关 (r=0.78,P<0.01)。结论 :NS大鼠伴随着尿蛋白排泄的增多 ,其尿IGF -1的排泄也增多 ,导致其血清IGF

Objective:To investigate the changes of insulin-like growth factor-1(IGF-1)in the serum,urine and liver tissue of adriamycin nephritic rats and to explore the pathogenesis of growth retardation in nephrotic syndrome.Methods:IGF-1levels in the serum,urine and liver tissue and24h urine proteins of both adriamycin-induced nephrotic syndrome(NS group)and control rats were measured.IGF-1levels were deˉtected by radioimmunoassay method.Results:Compared to control group rats,NS group rats showed signifiˉcantly reduced IGF-1concentrations in the serum(P<0.01),but significantly increased urine excretion of IGF-1(P<0.01).There was no statistical difference of IGF-1concentrations in the liver between two groups.The urine IGF-1level was negatively associated with the serum IGF-1level(r=-0.78,P<0.01),and positively associated with the24h protein excretion(r=0.78,P<0.01).Conclusion:In nephrotic rats,the urine IGF-1excretion increases with the increased urine protein excretion,resulting in decreased level of IGF-1in the serum.