桂枝汤对痹证(胶原诱导免疫性关节炎)小鼠肠黏膜免疫系统中CD_4^+、CD_8^+T淋巴细胞及SIgA的影响

Effect of Guizhi Decoction on CD_4^+,CD_8^+T-Lymphocytes and SIgA in Mucosal Immune System in Intestine of Mice with Bi Syndrome (Collagen In duced Immune Arthritis)

目的观察桂枝汤对痹证 (胶原诱导免疫性关节炎 )小鼠肠黏膜免疫学指标的影响。方法将80只雄性DBA小鼠随机分为 4组〔即正常组、模型组、桂枝汤高剂量组 (简称桂高组 )、桂枝汤低剂量组 (简称桂低组 )〕。模型组采用Ⅱ型胶原诱导性关节炎作为痹证模型 ,运用免疫组织化学染色法标记小鼠小肠及PP结中CD4 +、CD8+T淋巴细胞及SIgA数量 ,用图像分析仪进行统计计算其含量。 结果与正常组比较 ,模型组Ⅱ型胶原免疫后小鼠肠黏膜免疫系统中CD4 +、CD8+T淋巴细胞及SIgA数量明显减少 ,差异有显著性 (P <0 0 5或P <0 0 1) ,应用桂枝汤 (高、低剂量 )后 ,CD4 +T淋巴细胞及SIgA数量明显升高 ,但只有桂高组与模型组比较差异有显著性 (P <0 0 5或P <0 0 1)。结论桂枝汤可以增强痹证小鼠肠道黏膜免疫功能 ,从而可能诱导免疫耐受和免疫抑制。

ObjectiveTo observe the effect of Guizhi Decocti on (GZD) on immunological indexes in intestinal mucosal immune system of mice w ith Bi syndrome (collagen induced immune arthritis). MethodsEighty male DBA mice were randomly divided into 4 groups,the normal group,the model group and the high and low dosage GZD grou ps. Model of Bi syndrome was induced by collagen type Ⅱ. CD_4 +,CD_8 + T -lympho cytes and SIgA in mice' small intestine and PP node were labeled by immunohisto chemical staining and their number was calculated using imaging analyzer. ResultsCompared with the normal group,in the model gr oup,after immunization with collagen type Ⅱ,numbers of CD_4 + and CD _8 +T-lymphocytes and SIgA in mice' intestinal mucosal immune system reduc ed significantly (P<0.05 or P<0.01). After treatment with GZD of both high and low dosage,CD_4 + T- lymphocytes and SIgA increased significantly,but significant difference only sh owed in comparison between the high dosage GZD group and the model group ( P<0.05 or P<0.01). ConclusionGZD could enhance the immune function in inte stinal mucosa in mice with Bi syndrome,thus it might induce immune tolerance an d immune inhibition.