狼疮静颗粒对自发性狼疮小鼠肾狼疮样改变抑制作用的实验研究

Experimental Study on Inhibitory Effect of Langchuangjing Granule on Lupoid Change of Kidney in Lupus-prone Mice

目的探讨中药狼疮静颗粒对自发性狼疮 (NZB/NZWF1)小鼠狼疮样改变的抑制作用及其机制。方法应用狼疮静颗粒、泼尼松及狼疮静合泼尼松 3组药物 ,对 3月龄雌性BW狼疮模型小鼠进行干预治疗 6～ 12周 ,观察对各组小鼠病情发展动态变化、CD4 +、CD8+细胞和CD54 表达改变及对肾小球病理变化的影响。结果中西药物均能改善系统性红斑狼疮 (SLE)模型小鼠狼疮样改变的部分症状和血浆CD4 +、CD8+细胞分布 ,抑制血清细胞黏附分子 1(ICAM 1)含量升高和外周血淋巴细胞表面CD54 的高表达 ,抑制肾小球萎缩和系膜细胞的增殖 ,以狼疮静结合泼尼松组效果明显为优。结论狼疮静颗粒能有效控制并改善模型小鼠狼疮样发病及进程 ,并能调节模型小鼠细胞免疫功能 ,抑制过亢的免疫反应 ,对狼疮样肾炎的病理变化有改善作用 ,结合西药后可协同发挥疗效并显示出疗效优势。

ObjectiveTo study the effect and mechanism of La ngchuangjing Granule (LCJ) in inhibiting the lupoid change of kidney in lupus-p rone mice. MethodsIntervention therapy was applied to three group of BW female mice of lupus,3 months in age,for 6-12 weeks with LCJ,prednison e and LCJ+prednisone respectively to observe the dynamic development of disease ,changes of CD_4 +,CD_8 + and CD_ 54 expression,and the effect on pat hology of renal corpuscles. Results Both western and Chinese medicines can partially improve the symptoms and plasma CD_4 + and CD_8 + distribution,inhibit the increase of serum ICAM-1 con tent and the high expresion of CD_ 54 in surface of lymphocyte of peripheral blood,suppress the atrophy of renal corpuscles and the proliferation of mesang ial cell. The optimal effect was showed by the combination of LCJ and prednisone . Conclusion LCJ could effectively control and improve the genesis and development of lupoid changes in model mice,and regulate the cellular immune function,inhibit the ex cessive immune reaction,and improve the pathology of lupoid nephritis,it could co operate with western medicine to give full play of its effective role and show t he superiority in treatment.