芎芍胶囊对兔实验性动脉粥样硬化血管重构的影响

Effect of Xiongshao Capsule on Vasc ular Remodeling in Rabbit with Experimental Atherosclerosis

目的研究芎芍胶囊对实验性兔动脉粥样硬化 (AS)模型血管重构的影响 ,并探讨其可能的机制。方法采用 4F·Fogarty导管剥脱损伤腹主动脉内皮后继喂高脂饲料的方法 ,复制兔节段性AS模型。术后随机分为 8组 ,即模型 3天组、2周组、6周组 ,单纯内皮损伤组 ,普罗布考组 ,芎芍胶囊小剂量组、大剂量组及正常对照组。除正常对照组及单纯内皮损伤组外均予高脂饲料喂养 ,对腹主动脉病变最明显处标本作病理形态学检查 ,并利用计算机图像分析系统观察动脉损伤后血管重构相关指标的变化及药物对其的影响并进行内膜增殖与血管重构的相关性分析。结果球囊损伤腹主动脉内皮后 ,随时间延长 ,动脉内膜逐渐增厚 ,模型 6周组与模型 2周组及内皮损伤组比较差异有显著性 (P <0 0 1) ,芎芍胶囊大剂量与普罗布考组可明显减少内膜最大厚度 (MIT)及内膜面积 (IA) ,与模型 6周组比较差异有显著性 (P <0 0 1) ;随内膜增厚 ,动脉出现早期代偿性扩张 ,模型 2周组内弹力膜围绕面积 (IELa)、外弹力膜围绕面积 (EELa)及管腔最小直径 (MLD)、管腔面积 (LA)均较正常组明显增加 (P <0 0 1) ,至模型 6周时 ,随内膜进一步增厚 ,IELa、EELa反而有所缩小 ,血管代偿性扩张不足 ,管腔明显缩窄 ,MLD缩小 (P <0 0 1) ,各药物组均可增加MLD、LA 。

Objective: To study the effect of Xiongshao capsule (XSC) on vascular remodeling in porcine coronary balloon injury model. Methods: Coronary artery restenosis model was established by oversized balloon injury at mid region of the left anterior descending coronary artery. They were divided into 5 groups, untreated or treated with probucol, Xueguantong, low and high dose of XSC respectively, and compared. The effect of XSC on late lumen loss resulted from vascular remodeling was evaluated by quantitative histological method with combining histopathological analysis and coronary angiographic examination. Results: The (59±20)% of late lumen loss was caused by vascular remodeling and (41±20)% caused by intimal hyperplasia. Compared with the control group, all the treatment could significantly reduce the late lumen loss after balloon injury ( P <0.05 or P <0.01), both low and high dose of XSC could significantly reduce the late lumen loss caused by remodeling ( P <0.05 and P <0.01 respectively). Conclusion: Vascular remodeling plays an important role in late lumen stenosis formation in coronary artery after balloon injury. XSC could significantly inhibit the pathological vascular remodeling, and thus reduce the late lumen loss and prevent the restenosis of the injured coronary artery.