氯化汞诱导大白兔双相自身免疫性肾小球肾炎

AUTOIMMUNE AND BiPHASIC GLOMERULONEPHRITIS INDUCED IN RABBITS BY MERCURIC CHLORIDE

本实验采用日本纯种大耳白免、给予小剂量氯化汞反复肌肉注射,成功地制成双相自身免疫性肾小球肾炎模型,即免疫荧光可见IgG沿肾小球基膜(GBM)呈线样和颗粒状沉积两时相。病理改变为类狼疮性肾炎及膜性肾病二种组织学改变。该模型为进一步研究人类慢性汞中毒所致的自身免疫性肾损伤有重要的现实意义。

Japan rabbits were injected intramuscularly with mercuric chloride(MC) over prolonged time to test the hypothesis that MC can modify autologous antigens (or release autologous antigens) generating an autoimmune response antibody mediated nephritis. The results of studies showed that prolonged administration of small doses of MC frequently induces biphasic autoimmune glomerulonephritis characterized initially by linear IgG deposits along the glomerular basement membrane followed latter by granular IgG deposition.Two main changes of glomerular histology were observed by light and ectronm icroscope. The model appears as a good experimental model to study autoimmune disease and glomerulonephritis induced by mercuric chloride.