骨质疏松动物实验骨折风险性分析

Estimation of fracture risk of experimental animal osteoporosis model

目的 :评价骨质疏松动物模型发生骨折的风险性。方法 :健康雌性SD大鼠 3 6只 ,随机分为实验组和对照组各 18只。实验组切除双侧卵巢 ,对照组仅切开皮肤。于术后 2、 4、 8周两组处死 6只大鼠 ,作松质骨 (腰椎 )和皮质骨 (股骨干 )骨密度 (BMD)组织形态学和力学测定。结果 :与对照组相比 ,手术组松质骨BMD 2、 4、 8周均明显下降 ,2周皮质骨无明显差异 ,4、 8周则明显下降 ;股骨中段骨皮质厚度变薄 ;骨小梁体积 (TBV)占全部骨组织体积 (TTV)的百分比明显减少 ;成骨细胞和破骨细胞明显增多 ;股骨最大弯曲载荷。

Objective:To develop animal osteoporosis model and estimate the risk of fracture Method:Thirty six healthy female rats were randomly and equally divided and subject to kilateral ovariecfomy (DVX,18 rats) or sham operation (control,18 rats) group.Every 6 rats were killed at 2 weeks,4 weeks and 8 weeks after operation respectively,bone mineral density (BMD),histomorphometry observation and bending strength and compression strength measurement were performed in the cancellous bone (lumbar vertebra) and cortex(femoral shaft) Result:Compared with control group,osteepenia was well established in the OVX gruop In the OVX gruop,the BMD of cancellous bone was prominently decreased at 2 weeks ( P<0 01 ) 4 weeks and 8 weeks ( P<0 001 ),as for BMD of cortical bone,there was no difference between two groups,but decreased at 4 weeks and 8 weeks in OVX group ( P<0 001 ) The number of osteoblast and osteoclast was significantly incrcased;The ultimate bending load of femur and ultimate compression strength of vertebra marked decreased at 4 weeks and 8 weeks ( P<0 001 );Thenning of cortex of mod femoral shaft and declining of ratio between frabecula rolume and fotal bone rolume were observed