化疗对骨肉瘤患者淋巴细胞P170蛋白表达的影响及意义

Influence of chemotherapy on expression of peripheral blood lymphocyte P170 protein

目的 :观察化疗过程中骨肉瘤患者外周血淋巴细胞P170蛋白表达的变化及临床意义。方法 :应用流式细胞计技术检测 2 8例骨肉瘤患者化疗前后外周血淋巴细胞P170蛋白表达 ,以 2 0例健康献血者为对照。将其结果与骨肉瘤的检测结果相比较。结果 :骨肉瘤患者PBLP170蛋白表达率为 ( 2 3 96± 2 7 49) % ,明显高于正常对照组〔 ( 8 0± 7 15 ) % ,P <0 0 5〕。化疗前PBLP170蛋白阳性表达率为 42 84%。术前化疗组化疗后PBLP170蛋白表达率( 48 3 2 2± 3 6 5 74) % ,明显高于化疗前 ( 2 4 3 95± 2 5 3 92 ) % (P <0 0 0 1)。术前化疗前P170的阳性表达与骨肉瘤的TC NR无明显相关性。首次化疗前PBLP170表达率与预后无相关性。化疗前后PBLP170蛋白阳性表达率有明显差异 (P=0 0 0 1)。结论 :通过检测外周血淋巴细胞P170蛋白可以评价骨肉瘤多药耐药性在化疗过程中的变化 。

Objective:To study changes of expression of peripheral blood lymphocyte PBL P170 protein in osteosarcoma patients during chemotherapy Method:During chemotherapy,the expressions of PBL P170 protein were measured in 28 cases of osteosarcoma,20 normals were as the control The determined results were compared with the clinical outcomes Result:The expression rate of PBL P170 protein (23 96±27 49%) was much higher than that of the normal (8 0±7 15%, P<0 05 ) The positive expression rate of PBL P170 in pre chemotherapy osteosarcoma patient was 42 84% In pre operative chemotherapy group,the post chemotherapy expression rate of PBL P170 (48 322±36 574%) was much higher that of pre chemotherapy (24 395±25 392%)( P<0 001 ),the pre chemotherapy expression rate of PBL P170 was not relation to the post operative TCNR No relationship exited between the pre chemotherapy expression rate of PBL P170 and the prognosis of osteosarcoma No relationship was found between the pre and post chemotherapy expression rate of PBL P170 Conclusion:The measurement of PBL P170 can be used in evaluation of multi drug resistance changes during chemotherapy and to determine the clinical use of multi drug resistance reverse agents