大鼠不同部位软骨细胞的形态及表型特征比较研究

Comparison of the phentotyptic characteristics of intervertebral disc with cartilage cells from the same rat

目的 :比较研究大鼠椎间盘软骨终板和膝关节软骨的细胞表型特征的相关性。方法 :大鼠的软骨终板和关节软骨细胞分别予以消化培养。进行光镜、电镜观察其形态。使用免疫组化技术分别检测不同部位细胞的II型胶原表达。结果 :大鼠椎间盘软骨终板和关节软骨细胞形状相似 ,并且均表达II型胶原。结论 :本研究提示软骨终板表达软骨细胞的特征性胶原 。

Objective:To compare the morphology and phenotypes of cells extracted from the intervertebral discs of young rats with those of articular chondrocytes from the same animals Method:The intervertebral disc endplate as well as articular cartilage of rats were digested enzymatically The morphology of those cells were examined by light microscopy and transmission electron microscopys The special collagen II was determined by immunhistochemical analysis Result:The cartilage endplate cells were morphologically undistinguishable from ariticular chondrocytes All freshly extracted cells expressed collagen II Conclusion:Findings show that intervertebral disc endplate cells express cartilage specific matrix proteins,like the articular cartilage cells do