摘要
目的 评估外源性酸性成纤维细胞生长因子 (aFGF)治疗新生大鼠缺氧缺血性脑损伤 (HIBD)后学习记忆能力的改善。方法 建立HIBD新生大鼠模型 2 0只 ,随机分成aFGF试验组、生理盐水对照组 ,另取 10只新生大鼠作假手术组。各组于生后 32d开始进行Morris水迷宫测试 ,对其学习记忆功能进行动态观察。结果 对照组逃避潜伏期 (5 3.6 35± 4 7.30 0 )s较实验组 (2 8.36 5± 2 9.979)s和假手术组 (2 7.15 2± 30 .82 5 )s明显延长 (P <0 .0 5 ) ;拆除平台后跨越平台的次数对照组 (2 .5± 2 .6 77)次较实验组 (6 .1± 4 .0 4 0 )次和假手术组 (7.2±3.6 4 5 )次明显减少 (P <0 .0 5 )。结论 aFGF可恢复HIBD后大鼠的学习记忆能力 。
Objective To investigate the protective effect of exogenous acid fibroblast growth factor (aFGF) on cognitive dysfunction following hypoxic-ischemic brain damage(HIBD) in neonatal rats.Methods Twenty HIBD models in neonatal Sprague-Dawley rats were divided into two groups randomly:the aFGF trial group and NS control group. The other 10 rats were taken into the pseudo operation group. Each group′s spatial cognitive capability was evaluated by using the Morris water maze at the age of 32 days old.Results The latency to escape platform in control group (53.635±47.300)s was longer than that in trial group(28.365±29.979)s and the pseudo operation grou P(27.152±30.825)s(P<0.05);the frequency of pass through the platform in control group(2.5±2.677) was less than that in trial group(6.1±4.040) and pseudo group(7.2±3.645)(P<0.05).Conclusion The treatment with exogenous aFGF can improve the function learning and memory following HIBD, and the cognitive capability can reach normal level.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第5期383-386,共4页
Journal of Applied Clinical Pediatrics
基金
广东省科技厅
卫生厅联合攻关项目 [粤财 (2 0 0 1 ) 367-B30 50 2 ]
