血管内皮生长因子在佐剂关节炎大鼠滑膜组织中的表达

Expression of vascular endothelial growth factor in synovium of rats with adjuvant arthritis

目的 探讨血管内皮生长因子 (VEGF)在佐剂关节炎大鼠滑膜组织中的表达及其与关节病理积分关系。方法 通过右足垫皮内注射完全福氏佐剂建立关节炎大鼠模型 ,常规HE染色 ,计算关节病理积分 ,逆转录 聚合酶链反应 (RT PCR)检测VEGFmRNA表达 ,免疫组织化学染色检测VEGF蛋白表达 ,观察VEGF蛋白表达与关节病理积分关系。结果 模型组组大鼠滑膜VEGFmRNA及蛋白表达在 3、8周及 2 0周时均明显高于对照组 (P均 <0 .0 1) ,而且与关节病理积分呈正相关 (r =0 .984 ,0 .96 ,0 .92 9 P均 <0 .0 1)。结论 VEGF在关节炎形成及发展过程中起重要作用 。

Objective To investigate the expression of vascular endothelial growth factor(VEGF)in synovium of rats with adjuvant arthritis and the relationship between the histopathologic score and the expression of VEGF.Methods Adjuvant arthritis was established in Wistar rats by inoculating complete Freund's adjuvant(CFA). We calculated the arthropathologic score and the expression of VEGF mRNA and protein at different stages after CFA inoculation.Results In model group the arthropathologic score and expression of VEGF protein in synovium increased significantly all the time (P<0.01), and there were close association between the arthropathologic score and the expression of VEGF protein (r=0.984,0.96,0.929 P<0.01),respectively.Conclusion VEGF plays an important role in the progress of arthritis,which can directly affect the formation of new vessel of synovium.