低氧预适应增加小鼠脑组织内nPKCε膜转位

HYPOXIC PRECONDITIONING INCREASE nPKCε MEMBRANE TRANSLOCATION IN THE BRAIN OF MICE

目的 :初步探讨新奇型蛋白激酶C(novelproteinkinasesC ,nPKCs)在脑低氧预适应发生发展过程中的作用。方法 :利用蛋白电泳 (SDS PAGE)和蛋白印迹 (Westernblot)等生化技术 ,并结合本室已建立的小鼠低氧预适应模型 ,观察低氧预适应对小鼠海马和大脑皮层组织内nPKCs(nPKCε、δ、η、μ和θ亚型 )膜转位 (激活 )的影响。结果 :随低氧次数 (H0 H4 )或低氧耐受时间的增加 ,小鼠海马 (H0 :4 1.6 %± 1.4 %vsH1 H4 :4 6 .9%± 4 .5 % ,5 2 .7±3.9% ,5 8.8%± 2 .7% ,6 1.3%± 3.7% )和大脑皮层 (H0 :38.4 %± 4 .5 %vsH1-H4 :4 2 .4 %± 5 .0 % ,4 8.7%±6 .5 % ,5 5 .3%± 8.9% ,6 1.2 %± 10 .2 % )组织内nPKCε膜转位明显增加 ,且海马和大脑皮层分别在H2、H3、H4和H3、H4具有统计学的显著意义 (P <0 .0 1) ;而nPKCδ、η、μ和θ亚型在海马和大脑皮层组织内的膜转位变化均无统计学意义。结论 :nPKCε可能在脑低氧预适应的发生发展过程中发挥着重要作用 ,但需进一步的研究证实。

Aim: To explore the role of novel protein kinases C (nPKCs) in the development of cerebral hypoxic preconditioning. Methods: By using the mice model of hypoxic preconditioning, which was established before in our lab, the biochemistry techniques of SDS-PAGE and Western blot were applied to observe the effects of repetitive hypoxic exposure (H0～H4) on nPKCs (nPKCε、δ、η、μ and θ) membrane translocation in hippocampus and cortex. Results: nPKCε membrane translocation was increased in response to the hypoxic exposure times in the hippocampus (H0: 41.6%±1.4% vs H1～H4: 46.9%±4.5%, 52.7%±3.9%, 58.8%±2.7% and 61.3%±3.7%) and cortex (H0: 38.4%±4.5% vs 42.4%±5.0%, 48.7%±6.5%, 55.3%±8.9% and 61.2%± 10.2%) of mice, and there were statistic significances among H2, H3 and H4 in hippocampus, and H3 and H4 in cortex respectively(P< 0.01). But for nPKCδ、η、μ and θ membrane translocation, there were no any significant changes in hippocampus and cortex of hypoxic preconditioned mice. Conclusion: nPKCε may play an important role in the development of cerebral hypoxic preconditioning, but it need more evidence to prove.