摘要
目的 :研究三七总皂苷 (PNS)脂质体的制备方法并对其生理适应性进行初步考察。方法 :以薄膜分散法制备PNS脂质体 ,采用析因设计确定最优处方 ;以蟾蜍上腭纤毛摆动频率和大鼠肺水肿指数以及肺组织病理改变作为指标考察PNS脂质体的生理适应性。结果 :PNS脂质体为大单室脂质体 (LUV) ,圆形或椭圆形 ,外形圆整光滑 ,形态分布均一。平均粒径为 (15 4 6 .5± 32 1.4 )nm ,符合肺部给药对制剂粒径的要求 ,包封率为 (78.5± 2 .15 ) % ;PNS脂质体应用于蟾蜍上腭黏膜后 ,黏膜纤毛摆动频率是PBS的 92 .14 % ,PNS脂质体组大鼠的肺水肿指数和肺组织病理改变均显著轻于PNS溶液组 ,接近PBS组。结论 :PNS脂质体具有良好的生理适应性 。
AIM: To study the preparation and physiological compatibility of pulmonary administration with Saponius of Panax Notoginseng (PNS) liposomes. METHODS: PNS liposomes was prepared by thin film dispersion method. The prefered formulation was determined by factorial design. Ciliary movement in situtoad palate model and the changes of pulmonary edema index and pathology of lungs in rats were investigated to evaluate the physiological compatibility of PNS liposomes. RESULTS: PNS liposomes were proved to be large unilamellar vesicle (LUV) liposomes with spherical shape .The liposomes composed of PC and Ch has a high PNS capture efficiency of 78.5% ±2.15% . The mean vesicle size of liposomes were 1546.5nm ±321.4nm which were quite homogeneous in size distribution. The lasting time of ciliary movement after the rinsing of PNS liposomes was the 92.14% of the effect of PBS buffer. The pulmonary edema index and pathological changes of lung tissue in PNS liposomes group were significantly different from those in PNS solution group and close to those in PBS buffer group. CONCLUSION: PNS liposomes has good physiological compatibility with promising market development and application.
出处
《中成药》
CAS
CSCD
北大核心
2004年第5期352-356,共5页
Chinese Traditional Patent Medicine
基金
复旦大学研究生创新基金资助项目 项目类别:创业种子